The Experimental Research On The Change Of Glycometabolism To The Experimental Animal Model Of Spleen-Qi Deficiency Syndrome In Rat

ObjectiveIn order to research the change of glycometabolism to the experimental animal model of spleen-Qi deficiency syndrome in rat in this experiment.MethodsThirty rats were randomly divided into three groups: two types animal model of spleen-Qi deficiency syndromes in rat were set up by Rhubarb root laxation and Senna leaf laxation, and the Saline control groups .The rhubarb root laxation group was set up by 100% rhubarb root, and the senna leaf laxation group was set up by 20% senna leaf oral liquid, the control group was set up by Saline 0 The changes of the content of lactate acid, and the activity of succinate dehydrogenase(SDH) in the skeletal muscles and brain; the changes of blood sugar and the content of D-xylose in serum were observed. The change of the gene expression of glucokinase was observed by reverse transcription polymerase chain reaction (RT-PCR) in liver.Result1. Compared with the Saline control groups, growth of body weight on model rats of the rhubarb root laxation group and the senna leaf laxation group remarkably decrease (P<0.01).2. Compared with the Saline control groups, blood sugar on model rats of the rhubarb root laxation group and the senna leaf laxation group remarkably decrease (P<0. 05).3. Compared with the Saline control groups, the D-xylose content of serum on model rats of the rhubarb root laxation group and the senna leaf laxation group remarkably decrease (P<0. 01).4. Compared with the Saline control groups, the content of lactate acid (LD) in the muscles on model rats of the rhubarb root laxation group and the senna leaf laxation group remarkably increased (P<0.05).5. Compared with the Saline control groups, the activity of succinate dehydrogenase (SDH) in the muscles on modle rats of the rhubarb root laxation group and the senna leaf laxation group remarkably decrease (P<0.05).6. Compared with the Saline control groups, the content of lactate acid (LD) in the brain on model rats of the rhubarb root laxation group and the senna leaf laxation group remarkably decrease (P<0. 05).7. Compared with the Saline control groups, the activity of succinate dehydrogenase (SDH) in the brain on model rats of the rhubarb root laxation group and the senna leaf laxation group remarkably decrease (P<0.05).8. Compared with the Saline control groups, the gene expression of glucokinase in the liver on model rats of the senna leaf laxation group remarkably decrease (P<0. 01). Compared with the Saline control groups, the rhubarb root laxation group remarkably decrease (P<0. 05), compared with the rhubarb root laxation group, the senna leaf laxation group remarkly decrease (P<0.05).Conclusions1. The digestion, absorption and-metabolism of Glucose in the experimental rat model of spleen-Qi deficiency syndrome is abnormality.2. Glycometabolism of the skeletal muscles in the experimental rat model of spleen-Qi deficiency syndrome is abnormality, aerobic oxidation of glucose decrease and glycolysis reinforce, lactate acid (LD) of glycolysis product stack.3. Glycometabolism of the brain in the experimental rat model of spleen-Qi deficiency syndrome is abnormality, aerobic oxidation ofglucose and glycolysis decrease.4. The gene expression of GK in the experimental rat model of spleen-Qi deficiency syndrome decrease, glycogenolysis reinforce, glyconeogenesis reinforce, the intape glucose of liver and other constitution decrease, outpu glucose increase, prove that the glycometabolism in the experimental rat model of spleen-Qi deficiency syndrome is abnormality.5. The senna leaf and the rhubarb root set up the experimental rat model of spleen-Qi deficiency syndrome, the senna leaf is better.