Nephritis And Senility Fluid Observation, The Efficacy Of Slow Renal Failure Rats

Studying the function of preventing and curing of Shenyan Fangshuaiye on the chronic kidney failure through building the chronic kidney failure model of rats caused by 5/6SNX, and observing the weight of rats, the weight ratio of kidney and body, Blood pressure, 24-hour urine amount, 24-hour urine albumen , and biochemical index(blood serum creatinine, Blood urea nitrogen), and pathology stainings(H-E staining, Mallory staining ,amine silver staining).1. PurposeObserve Shenyan Fangshuaiye' s prevention and cure function on chronic kidney failure of rats that 5/6 SNX caused.2. Experiment material and methods2.1 Experiment animalSD male rats (offered by Beijng Weitonglihua Experiment animal technique limited company), weight: 180～200g, health.2.2 Experiment reagent and medicineNiaoduqing(offered by Guangzhoukangcheng department biotechnology technique limited company), Luodingxin(Beijing Nuohua department biotechnology technique limited company, authentication code: 05048), Shenyanfangshuaiye ( offered by the Chinese herbal medicine college, Beijing University of Chinese Medicine).2.3 Model building, group and treatFirstly, 90 SD male rats were divided into 3 groups at random, :normal group(9), false operation group(9) and operation group(72). Then operate and adaptability feed them for two weeks. Operation group mice were divided into 6 groups at random, including: Model group, the control group of Niaoduqing, the control group of Luodingxin , the low dose group of Shenyan Fanfshuaiye, the median dose group of Shenyan Fanfshuaiye, the heavy dose group of Shenyan Fangshuaiye. All the rats belong to normal group and false operation group were given 3 ml Physiology brine aevery day. While the others were given homologous drugs every morning. All the treatments lasted 15 weeks.