Intestinal Rehabilitation Apoptosis Of Human Colorectal Cancer

Purpose: To study traditional Chinese Compound Recipe Changfukang influence the apoptosis of human intestine large cancer cell and preliminarily explore its function mechanism.Method: (1) Model rats of transplantation tumor of human large intestinal cancer HT-29 were established. (2) The low, medium and high dose groups of Changfukang, the blank control group were set up. (3) The number of apoptotic cells and the gene expression of bcl- 2, caspase-3, TGF- p, bax in each group were quantitatively detected and compared by the application of immunohistochemistry and image analysis technique. (4) Statistical and analysis experimental data, Study traditional Chinese Compound Recipe Changfukang influence the apoptosis of human intestine large cancer cell and preliminarily explore its function mechanism.Result: Through detecte the apoptotic cells and find , each medicine group transplant tumours apoptotic cells increase than blank group, high dosage group transplant tumours apoptotic cells count remarkable rather than blank group and 5 Fu + CF chemotherapy group (P <0.01) . Indicating that changfukang has the function with better leading apoptotic of tumour on people's large intestine cancer HT-29. In addition experiment has find, important function albumen - "death protease " Caspase-3 and Bax, Bcl-2, TGF-β closelied related with to apoptotic of cell also, the content that Fas/Fasl organizes at the tumour uses medicine with changfukang the positive correlation change take place in the change of quantity. Indicate that changfukang transplants the expression of tumour Caspase-3 through promoting, increase the content ratio of Bcl-2, bax and TGF-P express and it raises to be may changfukang lead people large intestine naked mouse, HT-29 of cancer, transplant tumour cells function mechanism of apoptotic.Conclusion: Changfukang have its function of promotiong apoptosis of human large intestine cancer cell ,which may be correlated with change the expression situation of tumour organize bcl-2, caspase-3, TGF-P, Bax.