| Sj?gren's syndrome (SS) is a chronic exocrine autoimmune disease,characterized by dry mouth and dry eyes, B cell hyper activation andproduction of autoantibodies, and histologically by extensive lymphocyticinfiltration of salivary and lachrymal glands. The majority ofinfiltrating cells are T lymphocytes (55–75%), predominantly CD4+ T cells(45–55%) but also CD8+ T cells (15–35%). By now, the clear pathogenesis of SS is unknown. And there is littleeffective therapy against SS in modern medicine. Traditional Chinesemedicine is playing an important role in treating SS. Sheng Jin Kou Fu Ye is used as an effective Chinese medicine clinically.In order to provide the experimental basis for the immunological mechanismof SS and clinical using of Sheng Jin Kou Fu Ye, we have studied the effectsof Sheng Jin Kou Fu Ye on the immune function of SS model animals. 1 Establishing rats model and mice model of SS The SMG antigen of Lewis rats was purified by differentialcentrifugation. Then Lewis rats and C57bl/6j mice were sensitized onmultiple spots with SMG antigen in CFA. The HE stain showed that the model rats and model mice had moderatelymphocytes infiltration in substantial with the destruction of ducts. TheSMGs of the model rats were bigger than normal ones, while those of themodel mice were smaller than the normal ones. Besides, the salivarysecretion of SS model animals also was decreased, and the level ofdifferential antibodies in serum of SS model animals was higher than innormal animals. From these experimental results, it can be concluded thatthe rat model and mouse model of SS were successfully established. 2 The effect of Sheng Jin Kou Fu Ye on animal model of SS 2.1 Effect on the SS characteristic symptoms of SS model animals. Sheng Jin Kou Fu Ye could reduce the lymphocytes infiltration of SSmodel animals, alleviate the SMG swell of SS model rats, treat the SMGatrophy of SS model mice and promote the salivary secretion of modelanimals.英文摘要 V 2.2 The immunological function of Sheng Jin Kou Fu Ye on SS modelanimals Sheng Jin Kou Fu Ye could reduce the expansion of spleen and thymusgland of SS model animals, inhibit the proliferation of T lymphocyte ofSS model rats stimulated by ConA, constrain the proliferation of T or Blymphocyte of SS model mice stimulated by ConA and LPS. It also couldrestrain the DTH reaction induced by dinitrofluorobenzene (DNFB) of SSmodel rats and decreased the production of differential antibodies in theserum of model animals. 2.3 Effect on the Th1 like cytokine (IL-2, IFN-γ) and Th2 likecytokine (IL-4, IL-6) of SMG of SS model mice The results of RT-PCR showed that the mRNA transcription levels of IL-2,IFN-γ and IL-6 in SS model mice were higher than those in normal mice. Theresearch of ELISA showed that the protein secretion levels of IL-2, IFN-γand IL-6 in SS model mice were also higher than those in normal mice. ShengJin Kou Fu Ye could significantly decrease the expression of IL-2, IFN-γand IL-6 at both mRNA and protein levels. 2.4 Effect on the deficiency of Qi and Yin of SS model animal The changes of the weight, the consumption of food and the consumptionof water of the SS model animals were observed dynamically and the symptomof the deficiency of Qi and Yin was discovered in SS model animals. AndSheng Jin Kou Fu Ye could cure the deficiency of Qi and Yin. In conclusion, we researched the therapeutic mechanism of Sheng JinKou Fu Ye on SS model animals. The results show that Sheng Jin Kou Fu Yemight treat SS by inhibiting the enhancement of immune function of SS modelanimals and reducing the lymphocytes infiltration and inflammation in SMGof SS model animals. |
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