The Study Of The Expression Of P53 Gene And The Related Genes In Primary Sjogren's Syndrome

Objective To study the expression of p53 gene and the related genes in minor labial salivary gland of patients with primary Sjogren's syndrome (pSS).Methods Biopsies of minor submucosal labial salivary gland (SG ) were obtained from 30 patients with pSS and 10 control individuals. The immunohistochemical staining was used to detect the expression of p53, p21 and Bax.Results①The positive position of expression of p53 was mainly in nuclear, but some also were seen in the cytoplasm. Increased expression of p53 in pSS acinar and ductal epithelial cells (AEC and DEC) showed significant difference compared with that of control respectively(P<0.001, <0.001).②The positive position of expression of Bax was mainly in cytoplasm, the percentage of positive expression of Bax in pSS AEC and DEC were showed higher than in control, and it was significant difference between two groups(p<0.001).③The positive position of expression of Bax was mainly in nuclear, the positive expression of p21 in AEC show significant difference compared with that of control (P<0.01) ,and it showed no significant difference between in DEC and control (P>0.05).④Both in pSS AEC and DEC the expression of Bax had significant positive correlation with the expression of p53(P<0.01, 0.01).⑤There was significant positive correlation between the expression of p21 and p53 in pSS AEC(P<0.01), but there was no correlation between the expression of p21 and p53 in pSS DEC(P>0.05).Conclusions The increased expression of p53 in pSS SG epithelial cells may be hint the increased damnification of cell DNA, and the damnification of cell DNA may be induce the apoptosis through the expression of p53 and Bax. The apoptosis of cells not only result in the destruction of SG cells, but also may be one of pathogenesis of Sjogren's syndrome.