Gnat Menopausal Rat Ovarian Cell Apoptosis And Ultrastructure Of Experimental Research

Objective:The climacteric is the transitional period of a woman from bearing age to agedness. During this period, the body has very significant physical changes. Ovaries atrophy and dysfunction gradually. The excretion of estradiol (E2) decreased markedly and the vegetative nerve dysfunction gradually occurs. As a result, the patient may have many unhappy feelings such as hot, flashes, nervousness, depression, irritability, insomnia and so on, namely the Climacteric Syndrome. With the development of the society and the enhancing of life quality, the incidence of the disease take on a trend of increasing year after year. So how to prevent and cure Climacteric Syndrome effectively becomes a pop topic of medicine study. This report made a discussion of the effects of GNAT on the apoptosis and ultrastructure of ovary granulose cells of menopausal rat. It illustrated the possible mechanism of GNAT to cure the climacteric syndrome so as to supply the clinic application the basis of theory and experiment. Method:The study object was the female Sprague-Dawley (SD) menopausal rats of 11~15month.es old .The rats were divided randomly into 4 groups, i.e. GNAT groups, and estrogen groups, and menopausal contrast groups and with a normal groups (young rats of 4~6monthes old) as control. After 4 weeks, take the two ovaries each rat, the apoptosis expression was estimated by TUNEL, and Bcl-2 and Fas protein expression were estimated by immunohistochemical study. At the same time, we observe the ultrastructure of ovary granulose cells through electronic microscope. Result:1.The expression of ovary granulose cells apoptosis and Fas protein of the menopausal group increased evidently (P<0. 01), at the same time Bcl-2 protein expression decreased hugely than that of the young normal group (P<0. 01);2.After being treated with GNAT and estrogen, the expression of ovary granulose cells apoptosis decreased evidently than that of the menopausal group (P<0. 05); the differences of the above indexes between the two groups were not significant ( P>0. 05 ) ;3.After being treated with GNAT and estrogen, the expression of Fas protein decreased while Bcl-2 protein expression increased evidently than that of the menopausal group (P<0. 05); the differences of the above indexes between the two groups were not significant ( P>0. 05 ) ;4. GNAT and estrogen can improve the ultrastructure of ovary granulose cells. Conclusion:The results above indicate that GNAT can decrease the apoptosis in the ovary granulose cells, but also improve the ultrastructure of ovary granulose cells. Thismust be the probable action mechanism that GNAT to cure the climacteric syndrome. These evidences theoretically support the clinic application of GNAT.