Gnat Experimental Ovariectomized Role Of Prevention Of Postmenopausal Osteoporosis

Objective:Postmenopausal Osteoporosis (PMO) is syndrome shown on women caused by the functional descent on both ovaries and the level of estrogen. During the metabolism of their bones, the bone absorptive rate is higher than formative rate. As the result, the progression of calcium deficiency and the degeneration of bone tissue are the characters in this period. Clinical manifestations appear on these elder women, which are osteosclerosis and increasing the possibility of fracture. The approach used here is based on the former fundamental research. By using the molecular biology technique, IGF system was tested to study the mechanism of the GNAT prophylaxis and treatment the PMO. By conducting experiment, effects of different treatments were compared to see whether the difference exists, and provide theoretical evident on clinical manifestation prophylaxis and treatment.Methods:In this experiment, 60 unfertilized female rats were chosen as the experimental objects, whose ages were all five-month-old and weight from 200 to 250 grams. By doing oophorectomy operations on 40 rats, fake operations on 10 rats, and non-operation on the rest of them as a normal group, the rats were randomly classified into four groups, 10 rats in each group. They were ovariectomized group, estrogen group, GNAT prophylaxis group, and GNAT treatment group. GNAT prophylaxis group started to take medicine 7 days after the operations. Both GNAT treatment and estrogen groups were conducted medicine treatments 90 days after the operations. All medicine treatments were four-course treatments. After the last treatment, the rats were anesthetized to test BMC (Bond Mineral Content) and BMD (Bond Mineral Density) and taken blood by excising the rats' eyeballs. The blood was centrifugated to get about 1ml serum to test E2 (Estradiol) and IGF- I (Insulin-like Growth Factor I ) respectively by RIA (Radioimmunoassay) and ELISA (Enzyme-linked Immunoadsordent Assay).Results:1. Both BMC and BMD of ovariectomized rat's lumbar vertebra and thighbone decreased (P<0.01), so did the E2 and IGF-I (P<0.05);2. Comparing with ovariectomized group, GANT and estrogen can increase BMC and BMD of the lumbar vertebra and thighbone (P<0.05);3. Comparing with ovariectomized group, both GNAT and estrogen can enhance the level of serum E2 and IGF-I (P<0.05);4. Both GNAT treatment group and GNAT prophylaxis group can increase BMC and BMD of the lumbar vertebra and thighbone, E2 and IGF-1 of the serum. Nevertheless, the results were different maybe due to the time of taking medicine which caused different effects.5. According to relative analysis, the content of IGF-I and the E2 level of theserum show obviously positive relationship, r = 0.9001 .It may indicated E2 played the role by IGF- I on osteoblast.Conclusion:According to the above results, both BMC and BMD of the ovariectomized rat's lumbar vertebra and thighbone decreased, at the same time, E2 and IGF-I decreased evidently and showed obviously positive relationship. GNAT not only can help the ovariectomized rats to increase BMC and BMD of the lumbar vertebra and thighbone, but also to increase the EI and IGF-I contents to form bone cells and improve osteoporosis problem. Compared with estrogen, GNAT lowers the negative side-effect, provides the entire comprehensive functions of traditional Chinese medicine, and provides new theory on clinical treatment.