Correlation Of The Paraoxonase 192gln/arg Gene Polymorphism In Chinese Han People With Type 2 Diabetic Patients With Coronary Heart Disease

Objective: To investigate the association of paraoxonase 192Gln/Arg gene polymorphism with coronary heart disease in Chinese Han type 2 diabetes mellitus. Method: Paraoxonase 192Gln/Arg gene polymorphism was determined in 356 individuals matched by age and gender, including 80 patients with type 2 diabetes mellitus, 76 patients with coronary heart disease, 96 type 2 diabetic patients with coronary heart disease and 104 healthy subjects as controls. The paraoxonase 192Gln/Arg genotype was assessed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. Variables, including BMI, TC, TG, HDL, LDL, ApoAI, ApoB and paraoxonase 192Gln/Arg genotype were analyzed by multivariate logistic regression to determine the independent risk factors for coronary heart disease in type 2 diabetes mellitus. Result: The frequency of the paraoxonase 192Gln/Arg genotype and the frenquency of the R allele showed significant difference between type 2 diabetic patients with coronary heart disease and control subjects or CHD patients (P<0.05), but there was no difference in genotype frequencies or allele frequencies between type 2 diabetic patients with coronary heart disease and type 2 diabetic patients (P>0.05). The most frequent genotype and allele of 192 Gln/Arg polymorphism of paraoxonase gene in Chinese were genotype QR and allele R, but in European were genotype QQ and allele Q. Their frequency distribution showed significant difference between Chinese and European. The genotypes carried R allele were independent risk factors in diabetic patients with coronary heart disease. Conclusion: Paraoxonase 192Gln/Arg gene polymorphism is associated with coronary heart disease in Chinese Han type 2 diabetes mellitus.