| Objective To investigate association of paraoxonase gene 2 polymorphism with type 2 diabetes mellitus,diabetic nephropathy,diabetic retinopathy. Methods The genotypes of paraoxonase gene 2 polymorphism (C311S at exon 9) was determined by polymerase chain reaction-restriction fragment-length polymorphism analysis in 427 type 2 diabetic patients(2DM),73 impaired glucose tolerance patients(IGT), 113 normal control subjects(NGT) in Chinese people in AnHui region. Type 2diabetic patients were divided into three groups defined by urine albumin excretion rate which consisted of normoalbuminuria(l 11 ),microalbuminuria(l 52),clinical diabetic nephropathy(64).We also divided type 2 diabetic patients into three groups defined examination of dicret ophthalmoscopy which consisted of diabetes without retinopathy(145),with simple retinopathy(122) and with proliferate retinopathy(20). We compared the genotypes frequency and allele frequency among groups. Results we found no statically significant difference of genotypes frequency and allele frequency of PON2C311S gene polymorphism among 2DMJGT and NOT groups and among diabetic retinopathy groups. But there were statically significantly different genotypes frequency and allele frequency of C311S polymorphism in the normoalbuminuria and microalbuminuria (incipient nephropathy) .The CC+CS genotype frequency and C allele frequency were significantly higher in incipient nephropathy than in nomoalbuminuria group, (P=0.02, P=0.009). Multiple logistic regression suggested that C allele was an independent risk factor of incipient nephropathy(P=0.037). Conclusion These results imply that in Chinese people in AnHui region, PON2 C311S gene polymorphism is significantlyassociated with incipient nephropathy in type 2 diabetes. C allele may be anindependent risk factor of incipient nephropathy. We did not find enough association ofthe polymorphism with diabetic retinopathy and type 2 diabetes mellitus.
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