Acupuncture Mention Interpolation Reducing Method Of Blood Deficiency Model Rabbit Serum Ferritin, Total Iron Binding Capacity

As two basic reinforcing and reducing methods of acupuncture, lting-thrusting method and whirling method are widely used in clinic. There are still many different points of view about them:whether the reinforcing and reducing effect really exists, which factors affect the reinforcing and reducing effect, whether reinforcing and reducing effect has relation with the depth, angle, force and frequency of needle insertion. Because the subjectivity of acupuncture manipulation, it's hard to standardized in clinic and leads to low repeatability in experiments. Therefore, it is very important to establish the quantization of acupuncture manipulation, which will provide with a base for the clinical and experimental research in future.Section one:Literature SummaryThere are two literature reviews. The first article is involved in the study about antique literature and theories; the depth, angle, force and frequency of the lting-thrusting method; and the research profile about experimental study. Section two:animal experimentObjective:To observe the effectiveness of reinforcing and reducing by lifting and thrusting to blood deficiency rabitts:①To study whether the effect is different between lifting-thrusting reinforcement method and lifting-thrusting reduction method on blood-deficiency rabbits;②To study whether the effect of lifting-thrusting reinforcement method is better than lifting-thrusting reduction method on blood-deficiency rabbits;③To evaluate objectively acupuncture manipulation of lifting-thrusting reinforcement and reduction.Methods:60 Rabitts were divided into five groups randomly:Blank Group(Group A), Model Group (Group B), quiet stay needle group (Group C), reinforcing group(Group D) and reducing group (Group E),12 rabitts per group. Drew blood from central veins and central artery and ear margin vein of rabit ears except Blank Group, once per two days and 10 ml per time, totally 5 times, (the volume of withdraw blood is 10% of the whole body blood). Change their normal fed rules since the date of building model, with adequate food and restriction alternately. Drew periphery blood of the rabitts after model built 2 days,17 days and 32 days, test serum ferritin and total iron binding capacity of blood serum after Centrifugal. Data analyze in SAS software.Results:Results contrasting between pre-modeling and post-modeling1. There are no differences in the SF and TIBC indexs among the five groups before modeling.2. Contrast with pre-modeling, the SF index of Group B, C, D,E are sharply decreased, the TIBC index are obviously increased.3. After modeling, the SF and TIBC index of Group B, C, D, E are significantly different with the blank group at the same time, and there are no differences among the four groups. Therefore we could concluded that the model is successfully established.Results contrasting between pre-treatment and post-treatment1.2 days after treatment, there are no differences in the SF and TIBC indexs compared with pre-treatment every group, compared with the blank group, the SF index are still lower and the TIBC index are still higher in Group B, C,D,E.2.17 days after treatment:SF index:①Group D and E are much higher than pre-treatment, Group E is significantly higher than Group B (P<0.05), though there is no statistic difference between Group D and B, the SF index of Group D are also obvious because of the relatively lower basic index.②The SF index of Group C are higher than pre-treatment,it is also higher than Group B(not statistically), which showed Group C could increase the SF index, but not so obviously as Group D and E.③There are no statistic differences between Group D and E, which showed no difference between reinforcing and reducing effect for the SF index.TIBC index:①Group D and E are much lower than pre-treatment, and also much lower than Group B at this time.②The TIBC index of Group C are relatively lower than pre-treatment, it is also lower than Group B(not statistically), which showed Group C could decrease the TIBC index, but not so obviously as Group D and E.③There are no statistic differences between Group D and E, which showed no difference between reinforcing and reducing effect for the TIBC index.3.32 days after treatment:the SF and TIBC index of group B,C,D,E are obviously recovered, and no more significant differences compared with Blank Group. (P<0.05)。Conclusion:1. Acupuncture treatment could effectively increase the index of SF of blood loss rabitts.2. Acupuncture treatment could effectively lower the index of SF of blood loss rabitts.3. the treatment of reinforcing and reducing by lifting and thrusting is good for the index of decreasing SF and increasing TIBC of blood deficiency rabitts and better than quiet stay needle treatment.4. there is no difference between reinforcing and reducing by lifting and thrusting for improving the index of SF and TIBC, no difference statistically between them, so we can not draw a conclusion that the method of strongly thrusting and lightly lifting is better than and lightly thrusting and strongly lifting.