Gonghuan The Hemostatic Soup Gonghuan Bleeding Liver Stagnation And Blood Stasis Syndrome Serum Protein Expression

Objective:To deal with the treatment mechanism of Gonghuanzhixue soup treating metrorrhagia after women wearing IUD of liver depression and blood stasis syndrome from expression level of serum protein.Methods:Collecting abnormal metrorrhagia women's serum of before and after inserting IUD,and before and after treatment using Gonghuanzhixue therapeutic,and the normal women's serum after wearing IUD determining the effective serum 95 were divided into five groups, A group(serum samples pre-set IUD,normal menstruation after the set IUD)22,B group (serum samples pre-set IUD,abnormal menstruation after the set IUD)9,C group(serum samples after the set IUD,normal menstruation after the set IUD)13,D group(serum samples after the set IUD, abnormal menstruation after the set IUD,liver depression and blood stasis syndrome)41,E group(serum samples before and after treating metrorrhagia after women wearing IUD of liver depression and blood stasis syndrome) 10.Using SELDI-TOF-MS serum protein mass spectrums were obtained. Compared with the Biomarker Wizard software detecting serum protein mass spectrums, from Gonghuanzhixue soup before and after treatment of serum protein expression level changes,treatment-related proteins were identified, combined with bioinformatics,to explore its mechanism. Results:Through the treatment Gonghuanzhixue soup, many abnormal expression of the serum protein levels have been corrected and restored to normal.It was founded that charge-to-mass ratio were 22876.8 and 23480.9 were related to the treatment mechanism of Gonghuanzhixue soup.Conclusion:From retrieving the protein databases,Lin7b and Rab25 were possible related to the treatment mechanism of Gonghuanzhixue soup. Pointed out the direction for further research.