Effect Of Danchuanqi Granulas On Mmp-9 After Ami In The Rats

Objective:in order to study the mechanism and curative effectiveness of the DanChuanQI granulas on the AMI.We established on rats the acute myocardial inchemic models to study the change of the activity of MMP-9 in the necrosis.Methods:adult wistar rats were igated the left descending coronary artery,then being devided randomly into five groups:sham-operation;saline;tongxinluo;low-dosage,high-dosage group.The quantity of each group is ten.Parts of the necrosis zone in rats heart were selected for HE and immunohistochemical stained with HE to identify the ischemic or necrosis degree.Immunohistochemical method was used to measure the secretion of MMP-9.Results:the activity of MMP-9 in the necrosis zone showed an obvious increase.The latent MMP-9 were activated efter infarction,which resulted in the damage of the fibrillar network and dalitation of the left ventricle and ventricle remodeling.DanChuanQi granulas not only attenuates the activity of MMP-9 but also prevents collagen synthesis, decreases the total collagen in the necrosis zone at the stage after inchemia.Thus regressing the progress dysfunction of the infarction heart and protecting the ischemic myocardium.Conclusions:the DanChuanQi granulas can alleviate coronaryspasm and prevent ventricle remodeling.