The Influence On Calcitonin Gene-Related Peptide In Serum Of Danchuanqi In Rats With Acute Myocardial Ischemia

Objective:To observe the effect of Danchuanqi Granulas on calcitonin gene-related peptide in rats with acute myocardial ischemia and discuss the mechamism of Danchuanqi Granulas on treating the acute myocardial ischemia,providing theory for clinical application of Danchuanqi Granulas.Method:We divided the fifty wistar rats(male:female 1:1)into five groups.The high dose group and low dose group of Danchuanqi Granulas, comparison group of Tongxinluo,model comparison group and normal comparison group,ten rats a group.Every group had oral administration before the operated time of twenty four hours,twelve hours and half hour respectively,and gave operation on thirty minutes after the last oral administration.Excluding the normal comparison group we set up the acute myocardial ischemia model by the method of ligating the coronary artery of the rats on other groups,took blood from portal vein after thirty minutes on the base of the successful making model,determined the content of calciatonin gene-related peptide.Result:1.Compared with the normal comparison group,the level of calcitonin gene-related peptide in the high dose group and low dose group of Danchuanqi Granulas,comparison group of Tongxinluo and model comparison group is averagely degraded,the difference is significant(P＜0.05);2.Compared with the model comparison group,the level of Danchuanqi Granulas, comparison group of Tongxinluo is averagely heightened,the difference is significant(P＜0.05);3.The calcitonin gene-related peptide level in serum in the high dose group and low dose group of Danchuanqi Granulas has no significant difference with Tongxinluo comparison group(P＞0.05); 4.Comparing with the low dose group of Danchuanqi Granulas,the level of calcitonin gene-related peptide in serum in the high dose group of Danchuanqi Granulas has no significant difference.(P＞0.05)Conclusion:The mechanism of Danchuanqi Granulas on treating the acute myocardial ischemia is related to the level of calcitonin gene-related peptide.According to the experimental result,we can infer that Danchuanqi Granulas have the similar effect and mechanism with o Tongxinluo capsule on treating acute myocardial ischemia.