| Objective:By establishing the Acute myocardial infarction(AMI)model of small-ear pigs in southern Yunnan,the left anterior descending(LAD)of experimental animals was continuously ligated and 3 hours later,blood samples were reperfused at different time points and the myocardium was detected.Concentrations of Cardiac troponin I(Cardiac troponin I,cTn-I)and Cardiac myosin binding protein-C(cMyBP-C),compare the two proteins in the pig AMI model and ischemia-reperfusion model To study the role and significance of cMyBP-C in the diagnosis of AMI.Method:A total of 9 Diannan small-eared pigs for the experiment provided by Yunnan Agricultural University(male or female),weighing 30-40kg,were randomly divided into 3 groups before surgery.Group A(sham operation group):only open the chest and open the pericardium without ligating the coronary artery Group(n=1);Group B(ischemic group):Permanent ligation of the middle and distal LAD group after opening the pericardium by opening the chest(n=4);Group C(ischemia-reperfusion group):Opening the pericardium and ligating the middle and distal LAD The group will be opened after 3 hours(n=4).Blood samples were collected before surgery in the three groups;blood samples were collected for 24 hours after 10 minutes of opening the pericardium in group A,blood samples were collected continuously for 24 hours after ligation of the middle and distal part of LAD in group B,blood samples were collected continuously for 3 hours after LAD ligation in group C,and LAD was opened after ligation for 3 hours After the blood flow was restored,the timing was restarted and blood was collected for 24 hours.After standing at room temperature for2 hours,all blood samples were sent to the Central Laboratory of Yan’an Hospital Affiliated to Kunming Medical University,and centrifuged in a high-speed centrifuge for 20 minutes at 1000×g.The supernatant was transferred to an EP tube and stored at-80℃.Then use the porcine cardiac myosin binding protein C enzyme-linked immunosorbent assay kit and porcine cardiac troponin I enzyme-linked immunosorbent assay kit to determine the content of cMyBP-C and cTn-I in each sample;the experimental data obtained adopts SPSS 26.0 Statistical software for analysis.Results:1.Combining the electrocardiogram of experimental animals,postoperative cTn-I changes and pathological slices,successfully established the acute myocardial infarction and ischemia reperfusion model of Diannan small ear pigs;2.The concentration and time trend of cTn-I and cMyBP-C in each group(1)The concentration of cTn-I at each time point in group B was analyzed by single-factor repeated measurement analysis of variance.There were significant differences in the concentration of cTn-I at each time point,F(1.194,2.388)=785.185,P<0.001.The cTn-I concentration of experimental animals in group B was significantly higher than the preoperative cTn-I concentration at 6 hours to 24 hours after the coronary artery was completely ligated,and the difference was statistically significant(P<0.05);cTn-I was found in myocardial infarction 6 It started to rise after hours and still showed an upward trend to 24 hours.The concentration of cMyBP-C at each time point in group B was analyzed by single-factor repeated measures analysis of variance.There were significant differences in the concentration of cMyBP-C at each time point,F(1.794,3.588)=541.905,P<0.001.The concentration of cMyBP-C in the experimental animals of group B was significantly higher than that of cMyBP-C before the operation from 1 hour to 12 hours after the coronary artery was completely ligated,and the difference was statistically significant(P<0.05);cMyBP-C started 1 hour after myocardial infarction Appear,peak at 6 hours,and drop to baseline level at 12-18 hours.(2)The cTn-I concentration of experimental animals in group C used single-factor repeated measurement analysis of variance at each time point before and after operation.There were significant differences in cTn-I concentration at each time point,F(1.581,3.163)=1633.985,P<0.01.The cTn-I concentration of experimental animals in group C was higher than that of preoperative cTn-I at 2 hours,3 hours,4 hours,6 hours,8 hours,10 hours,and 12 hours after reperfusion,and the difference was statistically significant(P<0.05));cTn-I began to increase 5 hours after myocardial infarction(2 hours after ischemia-reperfusion),and still showed an upward trend to 15 hours after myocardial infarction(12 hours after reperfusion).The concentration of cMyBP-C at each time point in group C was analyzed by single-factor repeated measurement analysis of variance.There were significant differences in the concentration of cMyBP-C at each time point,F(1.546,3.091)=658.259,P<0.01.The concentration of cMyBP-C in experimental animals in group C was higher than that of preoperative 1 hour,2 hours,3 hours after coronary artery ligation,0.5 hour,1 hour,2 hours,3 hours,4 hours,6 hours,and 8 hours after coronary artery opening The concentration of cMyBP-C,the difference was statistically significant(P<0.05);cMyBP-C began to appear 1 hour after myocardial infarction,reached a peak 1 hour after reperfusion,and dropped to the baseline level 8-10 hours after reperfusion;Conclusions:1.Successfully establish a porcine acute myocardial infarction and ischemia-reperfusion model through surgical thoracotomy and ligation of LAD.2.cMyBP-C starts to rise 1 hour after acute myocardial infarction.Compared with cTn-I,cMyBP-C rises earlier and can be used as a myocardial marker for early diagnosis of AMI.3.The peak of cMyBP-C appeared early after reperfusion,suggesting that reperfusion can accelerate its degradation and release rate,and can provide certain guidance for ischemia-reperfusion injury and vascular recanalization. |
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