Expression Of Foxo1a In The Rat Model For Pcos And Explore The Possible Role Of Foxo1a In Pathogenesis Of Pcos

Part IBuilding a DHEA-induced polycystic ovaries in the rat model exhibiting both ovarian and metabolic characteristics of Polycystic Ovary Syndrome Objective:To build polycystic ovarian syndrome (PCOS) rat model induced by dehydroeplandrosterone (DHEA) and investigate serum hormonal changes in the ovarium and the serum blood sugar levels , insulin resistance levels changes between polycystic ovarian syndrome (PCOS) rat model group induced by dehydroeplandrosterone (DHEA) and control group .Methods: 21-day-old female Wistar rats were divided into two groups in pairs. The rats in group 1 (model group)were injected daily with DHEA oil for up to 20 days and the rats in group 2 (control group) injected with only oil at the same time. Ovarian weight, serum insulin levels serum Fasting blood glucose levels and sex hormone levels in rat blood of both groups were determined. Light microscopic and electronic microscopic examination were performed.Result: The ovarian weight in group 1 was greater than that in group 2 (P<0.05). The serum testeosterone were significantly higher in group 1 than those in group 2 (P<0.01), so as the fasting serum glucose (P<0.01) and fasting serum insulin (P<0.05). The value of FBG×FINS/22.5 was significantly higher in group 1 than that in group 2 (P<0.05) , The ovaria in group 1 showed multiple follicular cysts. Conclusions: The DHEA-induced PCOS rat models were similar to those of the patients with PCOS, and the IR was observed. the DHEA-induced PCOS rat models is successful.Part IIExpression of Foxo1a in the rat model with PCOS and explore the possible role of Foxo1a in pathogenesis of PCOS【Objective】To investigate the Foxola gene levels in the ovarium between the polycystic ovarian syndrome(PCOS) rat model groups induced by dehydroeplandrosterone (DHEA) and the control groups.Compare Foxola gene levels in the ovary with serum hormone levels to investigate relation between Foxola gene expression and serum hormone levels.【Methods】Ovaries were collected from polycystic ovarian syndrome (PCOS) rat model groups induced by dehydroeplandrosterone (DHEA) and the control groups. The proteins expression of Foxo1a were examined by immunohistochemistry.Granulosa cells were obtained ovaries and examined using RT-PCR. Compare Foxola mRNA expression in the ovary with serum hormone levels.【Results】The expression of Foxo1a protein in oocyte nests and primordial follicles were detected in the nuclei and cytoplasms of some oocytes.The main expression of Foxo1a protein were detected in the nuclei of the granulosa cells in the polycystic ovarian syndrome (PCOS) rat model groups,but the main expression of Foxo1a protein were detected in the cytoplasms of the granulosa cells in the control groups. Foxo1a protein levels of the granulosa cells in the polycystic ovarian syndrome (PCOS) rat model groups was greater than that in the control group (P<0.01).Results of additional RT-PCR showed that Foxo1a mRNA levels in the polycystic ovarian syndrome (PCOS) rat model groups was greater than that in the control group (P<0.01). Foxo1a mRNA expression in the granulosa cells is correlated positively with serum androgen but not the other serum hormones.【Conclusion】The abnormal express of Foxo1a is likely involved in the pathogenesis and development of PCOS.