Pre-menstrual Syndrome Model Rats With Liver-qi Depression Serum Neurotransmitters, Hormones, And Metabolomics Research

Objective:To copy the premenstrual syndrome rat model of liver qi depression syndrome and after intervention by the former Shu particles, through the study of premenstrual syndrome, liver qi depression syndrome serum neurotransmitters, hormones and endogenous small molecule metabolites, the changes in spectra process, as well as the card is closely related group of characteristics of metabolic and biological markers to explore the mechanism of rats induced Yu Shu granule treatment by the former mechanism of the disease permits.Methods:The binding method of modeling and used by the former Shu particles intervention, changes in behavior were investigated by use of mines, using radioimmunoassay of serum E2, FSH, LH, ACTH, COR, Ald changes, high-performance liquid chromatography Detection of DA,5-HT, the use of UPLC-Q-TOF-based technology combined with PC A model analysis of the metabolomics approach.Results:Compared with the blank group, model group rats were significantly lower behavioral scoring, serum ACTH, COR, DA levels increased significantly, E2, Ald,5-HT decreased significantly, making model treatment group and the relevant indicators to be significantly reversed improvement of serum in each group there were significant differences in metabolic profiling and preliminary characterization of PMS to determine the 21 liver qi depression syndrome endogenous biomarkers.Conclusion:The binding law was passed in response to interference with stress-induced rat neural regulation and hormonal regulation of emotional changes in the formation of a variety of mental and nervous-endocrine-metabolism disorder, premenstrual Shu particles can improve symptoms of rats on the neurotransmitter, endocrine hormones and metabolism disorders are apparent in vitro.