Objective Evidence Study On "Seeing The Disease Of The Liver, Knowing The Liver And Spreading The Spleen, Actualizing The Spleen First" Based On The Rat Model Of Liver Depression

"Golden Chamber" said "Strengthening the Spleen to Treat Liver Disease Since Liver Disease Transferring to the Spleen" mainly refers to the liver disease changes to the spleen,while maintaining the temper filling to less susceptible to external evil invasion.It is the specific embodiment of Zhang Zhongjing's theory of disease prevention and treatment,and it is also an important treatment for liver disease,Liver spleen method widely used in the clinical prevention and treatment of liver disease.Through the experiments of macro characterization,observe the rat weight,urinary D-xylose excretion rate,serum D-in gastrointestinal hormone and the liver function and the hypothalamic pituitary adrenal axis(HPA)plasma adrenocorticotropic hormone(ACTH)and corticosterone(CORT)content,to further explore the objective evidence of "liver the disease,known as liver Chuan spleen,spleen".Objective:Discuss the objective evidence of "Strengthening the Spleen to Treat Liver Disease Since Liver Disease Transferring to the Spleen",for provides a biological basis to interpretation the theory cure not ill of "Strengthening the Spleen to Treat Liver Disease Since Liver Disease Transferring to the Spleen" in "Golden Chamber",and for the rich and the development of traditional Chinese medicine therapy and therapeutic principle of prevention and treatment of chronic liver disease are useful scientific exploration.Methods:1.Reference to xu chronic bondage of methods duplicate liver depression model rats,divided rats into normal control group?model control group?the SiNisan group,the LiuJunzi Decoction group,the Chaishao LiuJunzi Decoction group A,the Chaishao LiuJunzi Decoction group B(14 days prior to the four SiNisan,after 14 days to Chaishao LiuJunzi Decoction),the Chaishao LiuJunzi Decoction group C(14 days prior to the Chaishao LiuJunzi Decoction,after 14 days to SiNisan).2.According to the kit colorimetric method,to measure D-Xylose concentration in after centrifugal rats urine,calculate the urine D-wood excretion rate.3.According to routine HE staining to observe rats pathological changes of liver tissue,gastric mucosa and duodenal tissue in each group with light microscope.4.Using Elisa method to measure the content of GAS?MTL?SS?CCK in serum.5.Using fully automatic biochemical analyzer to measure the content of ALT?AST?ALP?TBILI?DBILI?TP?ALB.6.Using Elisa method to measure the content of ACTH?CORT in plama.Result:1.After four weeks building mold,normal control group rats are under control;Model group rats appear typical symptoms of liver depression syndrome,such as bad mood into a depression,for is shining down and easy dropping,loose stool and etc.Each dosage group intervention rats are normal,a few seems bad mood.Compared with normal control group,the weight growth of the model group rats has decline,the difference has statistically significant(p<0.05);Compared with model group,the weight growth of the SiNisan group,the LiuJunzi Decoction group,the Chaishao LiuJunzi Decoction group A,the Chaishao LiuJunzi Decoction group B and the Chaishao LiuJunzi Decoction group C all increase,the difference has statistically significant(p<0.05);Among these,the weight growth of Chaishao LiuJunzi Decoction group C is the largest.2.Excretion rate test results of Urine D-xylose:Model group(15.94±0.31)compared with normal group(27.12±0.90)is decline,the difference has statistically significant(P<0.05).Other dosage group compared with model group,the difference all have statistically significant(P<0.05).the SiNisan group?the Chaishao LiuJunzi Decoction group A compared with the LiuJunzi Decoction group?the Chaishao LiuJunzi Decoction group B?the Chaishao LiuJunzi Decoction group C,each two have statistically significant(P<0.05).Among these,the Chaishao LiuJunzi Decoction group C improving efficiency is best.3.Test results of Gastrointestinal hormone:Model group compared with normal group,GAS?MTL?CCK were all decreased to some extent,SS content was increased,the difference has statistically significant(P<0.05).Compared with model group,the content of GAS?MTL?CCK are increase,SS is decline in the SiNisan group,the LiuJunzi Decoction group,the Chaishao LiuJunzi Decoction group A,the Chaishao LiuJunzi Decoction group B and the Chaishao LiuJunzi Decoction group C;the content of GAS?MTL?CCK is the largest,SS is the lowest in Liu Junzi Decoction group.4.Biochemical indicators Test results of liver function:Model group compared with normal group,AST?ALT?TBIL are increased,the difference has statistically significant(P<0.05).Other dosage group compared with model group,AST?ALT?TBIL are all improved,ALP?ALB?TP improving efficiency are unconspicuous.5.Determination of ACTH,CORT:Model group compared with the normal control group,the content of ACTH and CORT are increased,the difference was statistically significant(P<0.05);SiNisan group,Liujunzi Decoction group,Chaishao Liujunzi Decoction Group A,Chaishao Liujunzi Decoction Group B,Chaishao Liujunzi Decoction Group C compared with the model group,the content of ACTH and CORT were improved,Among these,the Chaishao LiuJunzi Decoction group C improving efficiency is best.6.Results of pathological observation:Compared with the normal control group,the model group has no obvious abnormal changes in pathological morphology of the liver,stomach and duodenum.The results show that the model only results in functional abnormalities,but also does not cause organic damage.Conclusion:? Biochemical:indicators of liver function can change in Liver depression syndrome rats;Accordingly,Liver depression syndrome rats gastrointestinal hormone index will also be changed.When prompt the liver function is injury,at the same time,gastrointestinal motor function will be affected by the corresponding.? The liver and spleen function is abnormal of 28 weeks of liver depression syndrome rats,but the pathological tissue did not see abnormalities.? The model of liver qi stagnation syndrome rats and biochemical indicators of liver function abnormalities,gastrointestinal hormone index will occur with the corresponding change,suggesting that liver function damage and gastrointestinal function will be affected accordingly.? The pathological change of liver,gastric mucosa and duodenum has no obvious abnormality in model group rats and drug administration group rats.? For the treatment of liver stagnation syndrome,SiNisan scattered group,the Six Gentlemen Decoction group,a group of Chaishao Liujunzi Decoction,Chaishao Decoction of six noble in group B,group C five six Chaishao Sijunzi Decoction group administered group of gastrointestinal hormones had different degrees of improvement.Especially the spleen six Chaishao Sijunzi Decoction group,six Jun Zi Decoction group C optimal effect,SiNisan scattered the worst effect.SiNisan scattered six group,Sijunzi Decoction group and Chaishao Decoction of six noble armour group,Chaishao Liujunzi Decoction groupoup B,group C five Shiba Roku Sijunzi Decoction group was administered group on the biochemical indexes of liver function to a certain extent,of which six Sijunzi Decoction group shigemi spleen Decoction group C,Shiba Roku optimal effect,SiNisan scattered improvement The worst effect.? When testing the HPA axis,SiNisan scattered group,the Six Gentlemen Decoction group,a group of Chaishao Liujunzi Decoction,Chaishao Decoction of six noble in group B,group C Shiba Roku Sijunzi Decoction in each group of HPA of the ACTH axis,the improvement of CORT were different,of which four Liujunzi Decoction group scattered effect is the best.The above results proved that the drug administration group which first emphasize the the spleen and then soothing liver and strengthening spleen have a most obvious therapeutic effect to liver depression syndrome,these can be the objective evidence to verify"Strengthening the Spleen to Treat Liver Disease Since Liver Disease Transferring to the Spleen".