Neurotransmitter In The Female Liver Depression Status, Hormone Experiments

Objiective: Producing depression of liver-Qi animal models and giving Chinese medicime-JingQing Capsule to interfere to study the change of neurotransmitter and breeding endocrine. Moreover to make a preliminary analysis for the reason of depression of Iiver-Qi leading to Female affective psychosis and for the mechanism of JingQing Capsule in preventing and curing female depression of Iiver-Qi.Method: The animal model was made by means of binding up rat's limbs and putting it into special moulds. Using JingQing Capsule as trial drug to smooth the circulation of liver-Qi. We observed the change of rat's general condition, spirit, behavior weight. Detecting the change of NE, DA, 5-HT in the rat brain stem by fluorospectiophotometry and the change of E2, P, T, PRL in the rats serum and B -endorphin in hypothalamus by radioimmunoassay. Detecting the content of ER in hypothalamus by immunohi stochemi cal method.Result: Some changes of the general condition spirit, behavior and weight of model rats occurred;The content of neurotransmitter abnormally rised up such as NE, DA, 5-HT and E2, P in the rats serum also rised up;ER and B-endorphin in hypothalamus both stepped up obviously. However above-mentioned targets appeared differential reversion in rats of the treated groop.Conclusion: The animal model of Iiver-Qi depression made by means of binding up is an ideal TCM animal model. Liver-Qi depression can lead to abnormality of neurotransmitter and estrogens So to cause diseases of psyche and genital system.