Researches On Speciation Analysis And Determination Of Trace Elements In Mongolian Medicine Xirawrel

The studies of determination and speciation analysis of trace elements in Mongolian medicine were reviewed in both domestic and abroad field. The meaning and content of this research work were represented in the first chapter.The Microwave-assisted digestion, a pretreatment method for the samples of Mongolian medicine, was conducted. The optimal condition for effective digestion of Mongolian medicine using Microwaves was confirmed by the trials of different powers, temperature and digestion times. The results showed that the compound digestion solution5mLHNO3+2mLH2O2+1mLH2O was most effective for liquid samples while the compound solution5mLHN03+1mLHC104+1mLH20was most efficient for solid samples under the pressures of (8、12、20) bar for digestion powers of (300、500、700) W for digestion times of (4、6、6) min, respectively, as was evidenced by the complete digestion of samples or clean solution using ladder-elevating temperature method. So, our data provides the optimal condition for pretreatment of samples for Mongolian medicine.The Mongolian medicine Xirawrel was digested using Microwave-assisted digestion and15kinds of trace elements were measured by ICP-AES. The result indicated that the orders of relative abundance of trace elements detected are:Ca>As>Al>Mg>Fe> Ni>Zn>Sr>Cu>Mn>Cr>Co>Pb>Ag>Cd, among them Ca reached the highest content of30521.18μ g/g. In Xirawrel, the content of beneficial elements such as Zn, Mg, Mn, Fe,Cu, Ca was higher, while the content of detrimental heavy metals such as Pb, Ag, Cd was lower with exception of As as its content reached15627.54μ g/g. Even if the content of As in Xirawrel exceeds state limit, it does not compromise the medicinal value of Xirawrel because it is still useful for special treatment of the patients in emergency. Recovery rate is97.5-108.5%and RSD is below2.16%in standard recovery rate experiment, demonstrating the high accuracy and precision of this method. Those results provide valuable data for the determination of relationships of trace element content and Mongolian medicine efficiency.Speciation analysis was performed on the trace elements from Mongolian medicine Xirawrel including Cd, Cr, As, Zn, Mg, Mn, Fe, Cu, Ni, Ca, Al, Go, Sr, Pb and Ag. Primary and secondary morphologic separations were carried out upon the basis of extraction using stilled water and artificial gastric fluid. The samples were digested with Microwave-assisted digestion after separation. The trace elements were measured by ICP-AES method and the speciation parameters were calculated.(1) Primary morphologic separation:soluble and suspended samples from water extracts and gastric extracts were separated by micro-porous membrane, and the speciation parameters were calculated.(2) Secondary morphologic separation:Organic and inorganic parts were isolated from soluble samples in two different media using macro-porous absorption resin column and the percentages in organic and inorganic states were calculated. Free-form and non-free-form parts were isolated from soluble samples in two different media using cationic exchange resin and the percentages in free-form and non-free-form states were calculated. Static and non-static parts were isolated from soluble samples in two different media using cationic exchange resin and the percentages in static and non-static states were calculated. The results showed that the extraction ratios of15trace elements in soluble water extracts were2.27%-0.14%while these ratios in artificial gastric fluids were2.39%-83.99%. The trace elements" like Cr, Zn, Mg, Mn, Fe, Cu, Ni, Ca and Sr existed in the free, non-static and inorganic states while the trace elements like As, Pb, Ag, Co and Cd existed in the non-free, static and organic states. Our data provides reference basis for the relationships of trace elements and medicinal efficacy and also for the recipe control and quality control of Mongolian medicine.The results provides the new reference and basis for the research of the relationships of trace element content and Mongolian medicine efficiency and the control of compound and quality of Mongolian medicine.