Preparation And Properties Of New Mongolian Medicine Gamuzhuer Nanofibers By Electrospinning

Mongolian medicine Gamuzhuer is a traditional external powder,which has remarkable curative effect on ulcer,erosion and other wounds and is widely used in clinical practice.At present,as a powder,it is not convenient to use,which affects the continuous efficacy of the drug and whose continuous efficacy need to be improved.In this study,the nanofibers loaded with Mongolian medicine Gamuzhuer were prepared by electrospinning technology,and the technological process and fiber properties were explored to improve the shortcomings of Gamuzhuer powder,which provide a basis for the development of new Gamuzhuer Patch.Firstly,the safe,non-toxic and biocompatible polyvinyl alcohol（PVA）and Mongolian medicine Gamuzhuer（Hereinafter referred to as M）were prepared into drug-carrying nanofibers by electrospinning methods,and the optimal ratio was determined as PVA:M=10:2.According to the scanning electron microscope image,the fiber diameter was186nm.Fourier infrared spectroscopy（FT-IR）of the powder and the drug-carrying fiber showed the same absorption peak at 689cm^-1,which preliminarily confirmed that the drug loading was successful.The optimal time of the glutaraldehyde cross-linked drug-loaded fiber was 120h.After the cross-linked fiber process,the water absorption rate of the fiber reached 379.67%,and the dissolution rate was 47.24%,while the diameter of the fiber was270nm,which effectively improved the fiber properties and laid a foundation for further development.Secondly,the skin irritation of the new Mongolian drug-carrying nanofibers was evaluated by rabbit in vitro experiment.The average skin reaction value after single administration was 0.125 points（P<0.5）,and the average skin reaction value after multiple administration was 0.033 points（P<0.5）.Finally,the results of tissue section showed that the new Mongolian medicine nano-fiber had no irritation to the skin.In vitro antibacterial experiments were conducted to analyze the efficacy of the new Mongolian drug-loaded nano-fibers.The results showed that the antibacterial rates of 0.5g new Mongolian drug-loaded nano-fibers（containing 80%of the drug powder）against Staphylococcus aureus and Pseudomonas aeruginosa could reach 100%and 97%respectively,which was the same as that of 0.1g drug powder.It was helpful to promote the healing of wounds such as erosion and ulcers.Then,gas chromatography-mass spectrometry（GC-MS）was used to detect the borneol in the drug-loaded nanofibers,and the systematic verification was carried out.A method for the detection of borneol in the drug-loaded nanofibers was established by GC-MS.The concentration of borneol measured by this method was linear in the range of 0.31～19.85μg/m L,and the specificity,sensitivity,precision,stability,repeatability and durability of this method were in line with the requirements of Chinese Pharmacopoeia.The average content of borneol in the new Mongolian medicine nanofiber was 291.9132μg/g.The method can be used for the determination of borneol in the fiber of new Mongolian medicine and provide data for its quality control.Finally,small molecule composition analysis was carried out on the new Mongolian medicine nanofiber and Gamuzhuer powder,and the small molecule composition in both of them was compared.Both of them were detected to contain 1092 small molecules in the positive ion mode and 498 small molecules in the negative ion mode.It contains phenylpropanoids and polyketides,organic heterocyclic compounds,flavonoids and other active components related to bacteriostatic,anti-inflammatory,and wound healing,in order to provide reference and basis for the quality control of the new Mongolian medicine nanofiber,and to provide data for the analysis of its pharmacodynamic mechanism.The new Mongolian medicine loaded nanofibers were prepared by electrospinning method which is simple,non-irritating to the skin,and has good antibacterial properties.Moreover,the original Mongolian medicine components were not lost during the preparation process.It provides an effective solution to the problems such as inconvenience of use of Gamuzhuer powder and inability of continuous administration.