Effects Of Zophobas Morio Protein On Exercise Tolerance And Related Physiological And Biochemical Indexes In Sports Training KM Mice

Zophobas morio belonging to Insecta, Coleoptera and Tenebrionidae is native to Central America, South America, the West Indies, and Latin America and other regions. It was imported from southeast Asian countries to China in recent years. Its high protein content made it became an excellent new type of resource insects.In this research, Z. morio was used as source of supplementary protein to mice after exercise. Three dose groups were set as high, medium and low (1.67 g kg-1 bw d,3.33 g kg-1 bw d and 6.66 g kg-1 bw d). The administration was carried out every day on quantitatively lavaged subjects over a total period of 30 days, then determined related indexes. The result indicated that:1. Effects of Z. morio protein on body weight and tolerance in sports training miceThe body weight of mice increased as the increase of supplement dose in 3 protein used groups; the highest body weight growth rate came from the group in which the highest dose was used and was heavier than low does group significantly. The pole-climbing time, exhaustive swimming time increased as the increase of supplement dose. The pole-climbing time was significantly increased in both high and medium dose groups. The exhaustive swimming time of each protein used group was longer than control group significantly.This showed that using Z. morio protein as protein supplement can increase body weight growth rate and raise the level of endurance in sports training mice.2. Effects of Z. morio protein on muscle in sports training miceThe leg muscle moisture, crude fat content in leg muscle of mice reduced as the increase of supplement dose. The leg muscle moisture in mice was significantly reduced in both high and medium dose groups compared with control group. The crude fat content in leg muscle of each protein used group has no significant difference compared with control group. The crude protein content in leg muscle of mice increased as the increase of supplement dose. The crude protein content in leg muscle of high does group increased significantly than control group.This showed that Z. morio was using as source of supplementary protein to mice after exercise can change skeletal muscle material proportion and promote the development of skeletal muscle.3. Effects of Z. morio protein on blood indexes in sports training miceLevels of red blood cell(RBC), erythrocyte mean corpuscular volume(MCV) and Red blood cell specific volume(HCT) of mice had no significant changes as the increase of supplement dose. Levels of hemoglobin(HGB), mean corpuscular hemoglobin(MCH) and mean corpuscular haemoglobin concentration(MCHC) of mice increased as the increase of supplement dose. Level of HGB in mice was significantly increased in both high and medium dose groups which compared with control group. Level of MCH in mice of high dose group was higher than control group significantly. Compared with control group, level of MCHC in mice was significantly increased in both high and medium dose groups.Levels of serum albumin(ALB), serum total protein(TP), serum urea nitrogen (BUN) and serum creatinine(CRE) of mice increased as the increase of supplement dose. Level of ALB in mice was significantly increased in high dose groups which compared with control group. Compared with control group, level of TP in mice was significantly increased in both high and medium dose groups. Level of BUN in mice was significantly increased in both high and medium dose groups which compared with control group. Level of CRE in mice of high dose group was higher than control group significantly. Levels of Creatine Kinase(CK), lactate dehydrogenase(LDH) of mice reduced as the increase of supplement dose. Level of CK and LDH in mice of high dose group was lower than control group significantly.This showed that supply of Z. morio protein to mice after exercise can provide substrate material for repairing and synthesis, it was good for the form of the corresponding functional protein, increasing protein catabolism. It also has obvious protective function for tissue damage caused by doing sport, and endurance was increased at the same time.