Effects Of Salt Stress On The Rubisco Carboxylase Activity In Alhagi Pseudoalhagi

In this experiment, legume Alhagi pseudalhagi and Vigna radiate as control, research of two kinds ofplant and analysis of Rubisco carboxylase activity under salt stress. The experimental materials wereplanted in vermiculite with greenhouse plastic pot cultured with1/2Hoagland’s nutrient solution, with0(control),50,100and200mM four concentration of NaCl treatment for12days, determination ofphotosynthetic gas exchange parameters and CO2response curve, carboxylation capacity of photosynthetickey enzyme Rubisco, cell membrane permeability of leaves, antioxidant enzyme activity index. Salttolerant plant Alhagi pseudalhagi and salt sensitive plant Vigna radiate photosynthetic capacity vary inresponse to salt stress. Pnand gsof Vigna radiate decreased with increasing salt concentration, Civalue hadno significant change. But the Alhagi pseudalhagi50mM treatment of photosynthesis was significantlyhigher than the control,145%,100mM treatment had no obvious difference with the control, the200mMis reduced to73%of control. Mild salt stress increased Pnand gs closely related to the low Civalue, downslightly, indicating that CO2assimilation rate enhancement of Pninduced. In addition, indicating that thechange of activity of different salt treated Alhagi pseudalhagi Rubisco enzyme, increased salt concentrationwas50mM when the Pnis increased by Rubisco enzyme activity; under100mM treatment, Rubiscoactivity is relatively low, so that the CO2assimilation rate had no significant difference from the control;200mM processing enzymes was limited. Although the carboxylation activity of Rubisco in50mM salttreatment of Alhagi pseudalhagi in vivo was higher than that of other treatments, but after the separationdetermined of activity is not significant difference with control. The results show that the Rubicco isseparated after the activity is suppressed.Under salt stress, Alhagi pseudalhagi leaf MDA content and cell membrane permeability increasesafter the first fierce easing, and the MDA content in Vigna radiate leaves and cell membrane permeabilityincreased with the increase of salt concentration and treatment time. The Alhagi pseudalhagi after saltstress SOD activity in leaves was higher than that of the control. Although the Vigna radiate processing inSOD activity than the control. The Alhagi pseudalhagi POD and CAT activity induction of increasedsignificantly in each treatment, and has strong ability of the decomposition of H2O2, POD and CAT activitydecreased in Vigna radiate. There fore, in the Alhagi pseudalhagi may rely mainly on the role of POD andCAT to Eliminate H2O2, and defense against oxidative stress. But the activity is no significant in Vignaradiate. SOD, POD and CATactivity of Alhagi pseudalhagi were induced by salt stress, the result show that,Alhagi pseudalhagi has strong ability to eliminate to H2O2than Vigna radiate.