Research On Numerical Simulation Of Micro-fluidic PCR Chip And Fluorescence Detection Micro-apparatus And Experimental Equipment

Miniaturized Total Analysis System(μTAS ) or Lab On a Chip(LOC) is the integrated biochemistry analyses laboratory, which integrates all of the process and step of biochemistry analyses and achieves the goal of functional integration and structure microform into a 100 millimeters of square retinue (or smaller) chip. It has outstanding features of high detecting speed, small sample dosages and high-throughput exchanging amounts. As a result, it attracted worldwide considerable attention.At present,the technical requirement forμTAS focuses on that the temperature control devices, sample introduction by column structure and fluorescence detection devices which are all functional-integrated and structure--microform into a micro-fluidic chip. A large amount research progress shows that the system which meets all of the requirements above has become a fascinating and urgent study field. However, the way to solve this problem is still in the primary stage in the world.According to above technical requirements in the temperature control and the fluid flow feature of PCR micro-fluidic chip, numerical simulation software is carried out to study which involve,(1) The entire temperature field of the polymer-based micro-fluidic PCR chip at three temperature zones temperature boundary conditions, and comparison of the different temperature distribution of heat-insulation layers with different air door holes as well as the different air cooling velocity.(2) The flow speed and pressure of the situation of liquid water flowing in a cycle in the micro-channel.As a representative microfluidic chip, PCR microfluidic fluorescence detection chip was studied in the following respects.Firstly, a prototype small-device for experimental fluorescence spectroscopy micro-determination has been obtained based on laser micromachining technology. Its experimental results show that: in pursuing the goal of "functional integration and structure microform", the approach and methods of this study is feasible and a series of new measures adopted are effective.Secondly, for effectively eliminating the interference from the stray light and the high-temperature environments in spectral detection, a light-blocking and heat-cooling technology was proposed to resolve the problem of heat dissipation in a avoid light and sealed container. In addition, this technology has been authorized by the national invention patent, which provides the technical foundation for studying the establishment of experimental apparatus and design verification of microfluidic PCR fluorescence detection chip, and solving the key technical problems. Finally, based on the existing research, an experimental apparatus aim for fluorescent PCR Microfluidic chip was designed and verified. This apparatus provide a technical basis for studying the three aspects of the "structural features microform" device research such as of temperature zoo, injection plunger and fluorescence detection, as well as quality testing of the fashioned chip production process in the future.