Experimental Study On The Effect Of Shenxian Shengmai Oral Liquid On The Treatment Of Slow Arrhythmia In Yangxue Syndrome

Purpose:Slow arrhythmia is a common disease and frequently encountered disease in diseases of the cardiovascular system, seriously affecting human health. Know about slow arrhythmia of traditional Chinese medicine focuses on palpitation, late vein disease related to discourse. Through clinical can see [1], this disease is shown more than early heart Yang(gas) performance, which in turn blood stasis and phlegm obstruction, long qi and Yin deficiency. So in this paper, combining the theory of TCM and modern medicine theory, through animal models experiment, observation the effect of Shengxian shengmai oral solution on the heart function,cAMP,cGMP, connexin45 and Na +-K~+-ATPase of heart Yang deficiency syndrome and slow arrhythmia in rats, and discussed its mechanism of action.Material and method:By propranolol lavage combined cold stimulation method to establish the heart Yang deficiency and slow arrhythmia model in rats. To join fairy litres of oral liquid of arteries and veins intervention:(1) measurement between groups of rats before and after treatment anus temperature, heart rate, and the cardiac output, left ventricular ejection fraction after treatment.(2) using immunohistochemical method, ELISA and inorganic phosphorus method, the determination of the content of each group rats serum cyclicAMP and cyclicGMP, Na~+-K~+-ATPase, myocardial Connexin45.(3) to observe the change of each laboratory indexes, evaluation model results and the impact of Shengxian shengmai oral solution on Connexin45 and Na +-k +-ATPase. Results: 1. Compared with the normal group, the model group rats of heart rate, anal temperature significantly decreased(P < 0.01), the left heart ejection fraction decreased(P < 0.01), decrease in cardiac output(P < 0.01), cyclicAMP content, cAMP /cGMP ratio decrease(P < 0.01), cyclicGMP content expression increased(P < 0.01), with statistical significance. 2. Compared with model group, traditional Chinese medicine in the rats heart rate, anal temperature increased significantly(P < 0.01), left heart ejection fraction increase(P < 0.01), increase in cardiac output(P < 0.05), cyclicAMP content increased(P < 0.05), cAMP /cGMP ratio increase(P < 0.01), cyclicGMP content decreased(P < 0.01), with statistical significance. 3. Compared with the normal group, the model group rats Na +-k +-ATPase activity decreased significantly(P < 0.01), the Cx45 relative grayscale significantly increased; Compared with model group, Chinese medicine group of Na +-k +-ATPase activity increased(P < 0.01), the Cx45 relative grayscale significantly reduced(P < 0.01), with statistical significance. Conclusion: 1. Objective indicators measured by experiment, to observe the symptoms of rats, drugs counterevidence, etc, were analyzed, and the use of propranolol combined cold stimulation method to establish the heart Yang deficiency type slow arrhythmia in rats model of success. 2. Shengxian shengmai oral solution can improve the rat anus temperature, improve heart function, increase the content of cAMP, the cAMP/cGMP ratio, reduce the cGMP content, antagonize slow arrhythmia caused by propranolol,makes the heart Yang deficiency syndrome to improve. 3. Shengxian shengmai oral solution can improve the Na +-k +-ATPase activity, the expression of connexin45, its mechanism may be through adjustment of Na +-k + atpase activity, the expression of connexin45, give play to the role of slow arrhythmia.