| The pharmacological effect of Chinese herbal medicine for activating blood circulation is mainly improve hemorheology, hemodynamics, microcirculation, antithrombotic, anticoagulant, anti atherosclerosis and myocardial ischemia, inhibiting inflammation, inhibit tumor etc.. Yixin Tongluo capsule is based on the theory of traditional Chinese medicine, on the basis of ancient, combined with years of clinical experience on the development of compound Chinese herbal preparation. The functions of tongluohuayu. The prescription application has obvious curative effect, wide use, which belongs to the clinical experience prescription, but the lack of modern pharmacological and toxicological basic research data. In this experiment, the theory of traditional Chinese medicine with modern pharmacology, pharmacodynamics research and investigation from the aspects of promoting blood circulation and removing blood stasis. This experiment through the pharmacological effects of a variety of experimental animal model to investigate the effects of Supplementing Heart Tongluo Capsule on promoting blood circulation to remove blood stasis, in order through efficacy studies, clear Yixin Tongluo Capsule pharmacodynamics and toxicity, clinical research for the future lay experimental basis, but also contribute to the future development of new drugs.Bioassay in the 2015 version of "the people’s Republic of China Pharmacopoeia stipulates that based on traditional Chinese medicine (TCM) activity for quality evaluation indicators, also known as bio identification or biological test is used to determine the biological and biological materials to foreign compounds stimulated the reaction, used to qualitatively the compounds are not active, or quantitative determination of dosage. Bioassay is biology, medicine, especially the important content and basic toxicology, in the process of new drug research in the bioassay plays a key role. The quality control of traditional Chinese medicine is an important direction for future research. According to the guiding principles of the pharmacopoeia in bioassay, the laboratory have explored the biological activity of traditional Chinese medicine injections of activating blood circulation to dissipate blood stasis method and the establishment of single herb medicine leech biological activity determination method, these will be included in the Pharmacopoeia of the people’s Republic of China, only promoting blood circulation to remove blood stasis Chinese herbal compound biological activity determination cannot be determined, the subject through the study of Yixin Tongluo Capsule chemical role in blood circulation, try to set up a method for the determination of the biological activity of promoting blood circulation to remove blood stasis Chinese herbal compound, explore the establishment of the effect of Yixin Tongluo Capsule on the active components in serum of rat bioassay. A new method for the study of blood circulation in vitro screening effect of compound traditional Chinese medicine, Chinese medicine to study the quality control and its clinical therapeutic effect.The main topics include the Yixin Tongluo Capsule pharmacodynamics test and blood circulation of the compound traditional Chinese medicine on biological assay of two parts:The first part of the efficacy of Yixin Tongluo Capsule promoting blood circulation and removing blood stasis testA, effect of Yixin Tongluo Capsule on acute blood stasis rat studiesMainly inspects the Yixin Tongluo Capsule on acute blood stasis model and blood stasis syndrome in TCM clinical more common, at present, the etiology of blood stasis syndrome have pathogenic cold, trauma, indignant, Qi deficiency, the main cause of acute blood stasis is sorrow anger and cold evil. There are many acute blood stasis model, such as cold coagulation and blood stasis, epinephrine, dextran, high fat cold coagulation, high-fat hyperlipidemia and blood deficiency and blood stasis six rats blood stasis model. This experiment according to the former experiments, preferred choice of adrenaline in acute blood stasis model, the model of acute blood stasis is furious state simulated epinephrine injection to the rat, with an ice water bath, the synthetic replication in rats with acute blood stasis model simulation of cold evil. The results showed that Yixin Tongluo Capsule in rats after 7 days of medication, blood coagulation index in all dose groups were significantly affected.Compared with Yixin each dose group and model group, TT significantly increased, Fib-c content decreased significantly. Therefore the Yixin Tongluo capsule can significantly affect blood stasis rats blood coagulation indicators, that is in the radix salviae miltiorrhizae, Radix Paeoniae Alba and leech on to the function of promoting blood circulation and removing blood stasis.B, Yixin Tongluo Capsule on pharmacodynamic study of rat arteriovenous shunt thrombosisThis paper mainly studied the influence of Yixin Tongluo Capsule on rat arteriovenous shunt thrombosis.Rat arteriovenous shunt thrombosis by in vitro passage of carotid artery and vein blood from the artery to vein Unicom, damage the vascular endothelial cells, activation of the coagulation system, caused the formation of thrombosis in vivo. Thrombus wet weight to dry weight decreased significantly, indicate that Yixin Tongluo capsule has the role of anti thrombosis, TT significantly extend the results show that Yixin Tongluo capsule can and thrombin interaction, inhibit the formation of thrombus through the inhibition of thrombin activity, with prolonged the coagulation time and anti thrombus formation.C, the efficacy of Yixin Tongluo Capsule on thrombosis in rats study of electric stimulationThis paper mainly studied the influence of Yixin Tongluo Capsule on thrombosis of electrical stimulation in rats.The mechanism of electrical stimulation of rat thrombosis is endothelial cell injury or dysfunction. This organ can endothelial cells secrete a variety of bioactive substances, plays an important role in maintaining the dynamic balance between coagulation and fibrinolysis system in. In general, the dominant is the anti coagulation function of endothelial cells, so as to maintain blood circulation. Promoting between coagulation and anticoagulation function balance change is endothelial cell damage or function disorder, will produce anticoagulation weakened or disappeared, coagulation function strengthen, finally thrombosis.D, effect of Yixin Tongluo Capsule on mice carrageenan model test study of black tailThis paper mainly studied the influence of Yixin Tongluo Capsule on mice and carrageenin tail vein.Carrageenan is extracted from seaweed containing sulfated polysaccharide substances, used in the preparation of a variety of inflammatory model and local inflammation and endothelial cell damage, in under the action of 1L-1B and lead to cell chemotaxis, infiltration and activation of the extrinsic coagulation system, which can cause blood clots, and in the formation of inhibition of fibrinolysis system. Test angle by subcutaneous injection of carrageenan, mouse tail vein for one-way vascular, injection after tail vein endothelial cell damage, resulting in thrombosis, forming a black tail. The results showed that Yixin Tongluo capsule can shorten the length of the black tailed and has significant anti thrombotic effect.E, effect of Yixin Tongluo Capsule on mice anti clotting effect studyThe main effects of Yixin Tongluo Capsule take effect of acid solution and coagulation time hypercoagulable animal hemorrhage on tanning. Ellagic acid is widely exists in the soft fruit, nuts and other plant tissues in a natural polyphenol composition, ellagic acid to chemical induced carcinogenesis and cancer had obvious inhibition, especially for colon cancer, esophageal cancer, liver cancer, lung cancer and other have good inhibitory effect. Ellagic acid can activate the endogenous coagulation system, increase the rate of platelet aggregation. Mouse tail vein injection of tanning flower acid solution is a comparatively simple a hypercoagulable animal model. The effects of the mouse tail bleeding time and coagulation time of change, showing the effects of Yixin Tongluo Capsule on hypercoagulable animal blood stasis role. The experimental results show that Yixin each dose group compared with model group, the bleeding time was significantly prolonged, the coagulation time was significantly shortened, showed that Yixin Tongluo capsule compound has significant anticoagulant effect.Determination of the second part of Yixin Tongluo Capsule medicated serum in vitro experimentA method for the determination of Yixin Tongluo Capsule medicated serum in vitro experimentThe main effects of Yixin Tongluo capsule containing serum affect anti coagulation in vitro. This paper selected rabbit as test animals, TT was chosen as important indicators of coagulation, drug concentration ratio is 1:determination of serum TT value, taking the drug concentration as the abscissa and TT values as ordinate and to make dose effect relationship curve and the linearity of the curve, the result shows that the correlation coefficient to evaluate. |
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