Study Of Flow Chemiluminescence Sensor Based On Molecularly Imprinted Polymer As Recognition Elements

The thesis is divided into two parts. Part one describes molecularly imprinting technology in detail and reviews recent development and application in analytical chemistry during recent ten years. Part two describes studies of chemiluminescence sensors based on molecularly (p -agonists) imprinted polymer as recognition elements respectively.Chemilumiescence (CL) is defined as the emission of radiation, usually in the visible or near infrared region, as a result of a chemical reaction. One of the reaction products is formed in an electronic excited state, and emits the radiation on falling to the ground state. CL analysis has been successfully and frequently used to many fields such as pharmaceutical science, environmental science and life science due to its many advantages such as high sensitivity, wide linear range, and simple and relatively inexpensive instruments. However the weak selectivity of the CL itself greatly limits its direct application to the complicated sample analysis. For improving the CL selectivity, researcher is working hard from two aspects: one is using CL combined with immune technology; two is using a separation technology such as liquid chromatography and capillary electrophoresis prior to CL detection. But these of methods not only require relatively expensive and complicated instruments, but also are greatly limited because of the incompatibility of separation conditions and CL reaction conditions in many applications.Molecularly imprinting technology (MIT) is a novel technology, which prepares the polymer that has special recognition and selectivity function for template molecule. Molecularly imprinted polymer (MIP) not only has excellent special recognition capacity compared with enzyme-substrate, antibody-antigen, receptor-ligand, but also has attractive features such as physical robustness, high strength, resistance to elevated temperature and pressure, and inertness towards acids, bases, metal ions and organicsolvents. Due to the high selectivity and stability of the MIP, MIP has been applied in several analytical fields such as sensor devices, chromatography separation, mimic enzyme, membrane, immune analysis etc. Combined the MIP with FIA-CL sensor, the CL selectivity and sensitivity would be greatly improved due to the special recognition capacity and binding capacity of MIP respectively, meanwhile the MIP--CL flow sensor shows the strong reversibility due to the CL reaction destroying the target structure in comparison with other sensors.1. Flow chemiluminescence sensor for determination of clenbuterol based on molecularly imprinted polymerIn this paper, a novel flow chemiluminescence (CL) clenbuterol sensor based on molecularly imprinted polymer (MIP) on line enrichment ng clenbuterol and chemiluminescence reaction of potassium permanganate and clenbuterol in the polyphosphate enhanced by formaldehyde. Clenbuterol in the urine was selectively adsorbed on the clenbuterol-imprinted polymer, which was packed into the flow cell. The formaldehyde and the polyphosphate with potassium permanganate flowed through the flow cell and reacted with the on line adsorbed clenbuterol and produced strong CL. The results show that the sensor was reversible. The relative CL intensity was linear with clenbuterol concentration from 1.0×10~-9g/mL to 5.0×l0~-8g/mL. The detection limit was 3.0×10~-10g/rnL (3). The R.S.D for 8.0×10~-9g/mL clenbuterol was less than 5% (n=7). The present method offered a high selectivity and sensitivity that made the quantitative analysis for trace clenbuterol (ng/mL) in the animal urine sample.2. Flow through chemiluminescence sensor using molecularly imprinted polymer as recognition elements for detection of salbutamolA novel flow through chemiluminescence (CL) sensor for detection of salbutamol based on molecularly imprinted polymer as recognition elements and the sensitization of CL reaction of luminol and ferricyanide potassium in the alkaline media has been developed. Salbutamol in the athlete urine sample was selectively bound by the salbutamol-imprinted polymer, which was packed into the flow cell and made up of the MIP-CL flow sensor by a very simple way.-The emerging stream of luminol and...