Preparation Of Histamine Molecularly Imprinted Polymer And Its Electrochemical Sensor

Histamine is one of the most common of biogenic amines,form decarboxylate of histidine by microorganism,intaking too much histamine will lead to histamine poisoning,so this study is aimed at using molecular imprinting technology in succession with electrochemical technology to build an electrochemical sensor which can detect histamine.This study selected histamine as template,2-?trifluoromethyl?acrylic acid as functional monomer to the preparation of molecularly imprinted polymer.For molecularly imprinted polymer,the ratio of template-monomer is important for the ultimate effects on the elution and adsorption properties of the polymer.This study first choice for different templates-monomer ratios by using the method of quantum computing chemical for complex system binding energy.Among them the study based on the DFT-B3 LYP method,with large polarization basis set 6-311+?d,p?are calculated optimal configurations for histamine and 2-?trifluoromethyl?acrylic acid respectively.The different ratios 3-d conformation of compounds were built and optimized the structure and binding energy with eliminating the BSSE by counterpoise procedure in Guizhou university cloud computing platform.Eventually found that 1:5 has the lowest energy?3130.15922226 kJ/mol?,more than the ratio due to molecular weight beyond limits to the output.Based on the theoretical calculation results,the direction of this research and choose the ultraviolet spectrum technology for further research.After the first determination of histamine and 2-?trifluoromethyl?acrylic acid this research found that absorption peaks appear at 244 nm and 245 nm,respectively.By the templatemonomer ratio from 1:1 to 1:10 assembling system for differential UV scanning,this research found that the stable compound can be built by 1 histamine molecule with 3 2-?trifluoromethyl?acrylic acid molecules at least.The conclusion can support computational chemistry results and through natural bonding charge of template molecule and functional monomer molecules structure analysis results.Based on the results of the study and the different ratios of assembling system for UV scanning,this research found that when the templatemonomer ratio reached 1:6 there is an obvious absorption peak cut,it illustrates the template and monomer to produce the most stable structure and hydrogen bonding sites.Therefore,the study will eventually focus on the ratio of 1:6 and 1:5,1:7 to build electrochemical sensor.Based on previous research,this study builds the three kinds of electrodes to characterize.Because of the HA is non-electrochemical active substance,so it cannot be directed by the electrochemical sensor and we bring in the K₃[Fe?CN?₆] to be a probe to characterize.This research polymerized 3 kinds of MIPs on the detection zone of carbon electrode and detect the elution time and reabsorption time were 4200 s and 3000 s of 1:5 by DPV.And the elution time and reabsorption time were 4200 s/3000 s of 1:6 and 3600 s/1800 s of 1:7.Then CV was used to detect the current response performances of different ratios of electrodes,the results showed that current volatility of 1:7 is unobvious,the elution performance of 1:5 is fine but the resilience in reabsorption is bad.So,the 1:6 is the best ratio because of the steadiest current response performance.Then this research detected that 1:6 electrode has a preferable anti-noise ability and detection range is 1⁴ ?mol/L,the detection rate is 97.6%¹01.6%,the detection limit is 0.8882 ?mol/L.In this article,a kind of HA-MIPs were polymerized on the detection zone of carbon electrode,and established a MIP-electrochemical sensor with preferable anti-noise ability.It is of great significance on rapid detection of histamine.