A Novel Chemiluminescence Sensor Array Based On Molecularly Imprinted Polymer

Molecular imprinting is a generic technology, which introduces recognition properties into synthetic polymers using appropriate templates. The method involves formation of a complex between a given target (template) molecule and functional monomers in appropriate solvent which is fixed by polymerisation into a growing polymer chain. Subsequent removal of the template leaves binding sites within the polymer possessing both shape and the correct orientation of functional groups, which are capable of selectively recognising the imprint species. Due to their predetermination, specific recognition and practicability, MIPs have been largely put into use especially in sensor.The combination of MIP and CL shows the advantage of them. The molecularly imprinted polymer (MIP) was employed based on its selective recognition and capture capabilities. The target analyte was separated from coexisting substances and selectively adsorbed on the molecularly imprinted polymer (MIP) microspheres, then was detected by peroxyoxalate chemiluminescence (PO-CL) reaction. Recently, we have developed an array sensor based on chemiluminescence (CL) imaging assay combined molecularly imprinted polymer (MIP) recognition. This method required the nonfluorescence analyte labled with fluorescent dye before determination, and it also need the labled analyte as template molecule for synthesizing molecularly imprinted polymer (MIP). The application of such sensors is limited, because pure analyte labled with fluorescent dye is expensive and difficult to obtain.In this paper, we conceive to recognize dansyl-analyte by molecularly imprinted polymers (MIPs) which were synthesized using precipitation polymerization with analyte as template, then determination by peroxyoxalate chemiluminescence (PO-CL) reaction. So, we devise a novel MIP-CL array sensor. This paper consists of two parts.Part one:reviewThis review summarizes molecular imprinting technique, the method of synthesiz, MIPs' applications and recent research of MIP-CL sensor.Part two:research section1. A novel chemiluminescence array sensor based on molecularly imprinted polymer and the application for the detection of glycine A new molecularly imprinted polymer (MIP)-chemiluminescence (CL) detection approach towards recognition of dansyl-glycine (Dns-Gly) is presented. The polymer microspheres were synthesized using precipitation polymerization with glycine as template and immobilized in microtiter plates (96 wells) using poly (vinyl alcohol) (PVA) as glue. The analyte (dansyl-glycine) was selectively adsorbed on the MIP microspheres. After washing, the bound fraction was quantified based on a dansyl-glycine-bis-(2,4,6-trichlorophenyl) oxalate (TCPO)-H2O2 chemiluminescence reaction enhanced by imidazole. The cavity of MIP synthesized with glycine as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-glycine.2. Molecularly imprinted polymer-based chemiluminescence array sensor for the detection of prolineA new molecularly imprinted polymer (MIP)-chemiluminescence (CL) method has been developed for detection of proline. The molecularly imprinted polymer microspheres were synthesized using precipitation polymerization with hydroxyproline, a structural analogue, as the template. Polymer microspheres were immobilized in microtiter plates (96 wells) which selectively adsorbed the analyte (dansyl-proline). After washing, the bound fraction was quantified based on peroxyoxalate chemiluminescence (PO-CL) reaction enhanced by imidazole. The cavity of MIP synthesized with hydroxyproline as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-proline. The MIP-CL method can become a useful analytical technology for determination of proline in real sample.3. Molecularly imprinted polymer-based chemiluminescence array sensor for the detection of clenbuterolA new molecularly imprinted polymer (MIP)-chemiluminescence (CL) method has been developed for detection of clenbuterol. The molecularly imprinted polymer microspheres were synthesized using precipitation polymerization with clenbuterol as the template. Polymer microspheres were immobilized in microtiter plates (96 wells) which selectively adsorbed the analyte (dansyl-clenbuterol). After washing, the bound fraction was quantified based on peroxyoxalate chemiluminescence (PO-CL) reaction enhanced by imidazole. The cavity of MIP synthesized with hydroxyproline as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-clenbuterol. The MIP-CL method can become a useful analytical technology for determination of clenbuterol in real sample. 4. Molecularly imprinted polymer-based chemiluminescence array sensor for the detection of lysineA new molecularly imprinted polymer (MIP)-chemiluminescence (CL) method has been developed for detection of lysine. The molecularly imprinted polymer microspheres were synthesized using precipitation polymerization with lysine as the template. Polymer microspheres were immobilized in microtiter plates (96 wells) which selectively adsorbed the analyte (dansyl-lysine). After washing, the bound fraction was quantified based on peroxyoxalate chemiluminescence (PO-CL) reaction enhanced by imidazole. The cavity of MIP synthesized with lysine as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-lysine. The MIP-CL method can become a useful analytical technology for determination of lysine in real sample.