Preparation And Application Of Molecularly Imprinted-chemiluminescence Sensors

In this paper, magnetic nanoparticles were prepared by coprecipitation technology,which surface modification with oleic acid, getting good dispersed in the organic phase andthe surface magnetic particles with a double bond. Combining the surface molecularimprinting technique, three kinds of phenolic molecularly imprinted polymer were prepared,with modification of magnetic nanoparticles as a carrie, acrylamide as functional monomer,ethylene glycol dimethacrylate(EGDMA) as cross-linker and2,2-azobisisobutyronitrile(AIBN)as initiator. The obtained polymer was characterized by the X-ray diffraction (XRD), infraredand scanning electron microscopy (SEM), and the adsorption performance were studied by uvspectrophotometry. Then，the molecularly imprinted polymers were used in flow injectionchemiluminescence analysis, constructing molecularly imprinted chemiluminescence sensorsand being used into the samples detected analysis．There were five parts in this thesis．In the first part, the development and present research status of the molecular imprintingtechnology and molecularly imprinted polymers was reviewed．And then, the concept andbasic principle of chemiluminescence was introduced. Finally the research progress ofmolecularly imprinted chemiluminescence detection was summarized.In the second part, an innovative flow injection chemiluminescence (FI-CL) sensor fordetermination of bisphenol A using magnetic oil-based surface molecularly imprintedpolymers (MIP) as recognition element is developed. The sensor provided a wide linear rangefor bisphenol A of1.0×109to2.0×108g/mL with a detection limit of7.1×10-10g/mL. Theproposed method was applied to determine bisphenol A in baby bottles and cups and obtainedsatisfactory results.In the third part, an innovative flow injection chemiluminescence (FI-CL) sensor fordetermination of acetaminophen was constructed by the same technology and the conditionswere optimized． This method sets the chemiluminescence of the advantages of highsensitivity and high selectivity of molecular imprinting. The linear range for acetaminophenwas1×10-10to8×10-9mol/L with a detection limit of2.5×10-10mol/L. The proposed methodwas applied to determine acetaminophen in samples and obtained satisfactory results. In the fourth part, synthesizing hydroquinone magnetic surface molecularly imprintedpolymer, an innovative flow injection chemiluminescence (FI-CL) sensor for determination ofhydroquinol is developed. the conditions were optimized．The sensor provided a wide linearrange for acetaminophen of2×10-10to1.0×10-8g/mL with a detection limit of7.9×10-10g/mL.The proposed method was applied to determine acetaminophen in samples.In the fifth part, a new method was developed to determinate benzoic acid. The linearrange was of2×10-9to8.0×10-8g/mL, the detection limit was5.4×10-9g/mL. The relativestandard deviation (RSD) for the determination of1.0×108g/mL benzoic acid was2.7%(n=11). As a result，the new method had been applied to the determination of benzoic acid inreal samples with satisfactory results．...