Integrated Capillary Electrophoresis Chip Sample Injection Numerical Simulation

Integrated capillary electrophoresis analysis system with its advantage of fast, multifunctional, high-efficiency and can be deal with computer is a new microanalysis instrument in microanalysis area. It has got extensive application in DNA array analysis, DNA separation, PCR separation and analysis, cell separation and other biological and medical fields.The main problem in electrophoresis separation is how to push the sample liquid into the separation channel. Nowdays, the method to solve this problem is to use the electrokentic sample injection to control the flow mode of the liquid in the micro channel. When the solution and the chip has been fixed, the external potential will be the main factor to determine the flow mode. If the computer numerical simulation can get the potential, velocity vector and stream distribution, the computer then can simulate the liquid's flow mode in the micro-channel. It will improve the the experiment efficiency and offer some valuable clews to design the electrophoresis chip.The electrokentic sample injection system model has been founded in theory, and the rule of how the potential to control the liquid has been studied. A numerical model has been founded with Finite difference method, and it has been solved with Vorticity-flow function method. Another model has been founded with Control-volume method, and it has been solved with the Semi-Implicit Method for Pressure-Linked Equation Revised. The potential, velocity vector and the stream distribution in the micro channel have been got, and the stream distribution will reflect obviously the liquid's flow mode in the micro channel. The sample injection and separation process have been simulated with the second numerical model, and the normal rules of how the external potential to control the liquid in the micro channel has been concluded in theory, which helps to get the better potential to be used in the electrokentic sample injection process. These simulation relults help to design the pinched injection experiment, and the experiment result inosculates well with simulation result, therefor it validates the computer numerical model in this paper.