The Study About The Blood Lymphocytes Apoptosis Of The Free Combat Athletes After Different Training Load

Research purposesExcessive training can cause different visceral organs injury or fatigue. How to prevent exercise fatigue and injuries is the research focus of current sports medicine. This study chooses free combat training model, uses different training methods, and focuses on the occurrence, development and variation characteristics of immune function and the peripheral blood lymphocyte apoptosis of free combat athletes. It discusses the influence on immune function and the peripheral blood lymphocyte apoptosis of different training load and explores the cell biology mechanism that excessive training causes immunosuppression and lymphocytes reduction. The study applies flow cytometric to test the quantities of CD3+,CD4+,CD8+ and the apoptosis condition of peripheral blood lymphocyte after different training load. The author aims to find out the athletes'degree of exercise-induced fatigue, adaptation to training, and recovery condition according to the rangeability of immune function and peripheral blood lymphocyte apoptosis index after training. The thesis further explores the mechanism of lymphatic apoptosis caused by sports training, and the influence of exercise training on various cellular immune indexes T lymphocyte such as subsets, natural killer cells and natural killer T cells. To grasp athletes'adaptation to training intensity and changes of physical function in time can provide an objective basis for scientific sports training. The study is meant to find the possible mechanism that causes the occurrence and development of lymphocytes apoptosis and strives to explain possible mechanism exists between the changes of immune function and peripheral blood lymphocytes apoptosis from the molecular level.Research methods The article choices 12 male free combat athletes (healthy, height 176.3±7.3cm, weight 71.3±14.9kg, age 21.7±2.05years old, sports training period6.8±2.0years old) in Shanxi Province prepared for 2009, 11 games as subjects. Normal control (adaptation relatively quiet) group (group A), medium load group (group B), big load group (group C) and drop weight group (group D). The study uses training period and telemetry rate to determine the athletes'load, then selects 140-160 times per minute for medium load training athletes, above 180 times per minute for big load. The training mode is―521‖(medium load),―821‖(big load) playing sandbags (or circle bundle) and the two combat against mode. "521" refers to athletes carring five groups of special playing sandbags (or circle boundle) training, two minutes exercises and rest one minute for each group; "821" refers to the athletes carring eight groups of playing sandbags (or circle boundle), e two minutes exercises and one minute rest for each group; combat against refers to two athletes combating directly, five minutes exercises for each group and two minutes rest between three groups. Finally extract 2ml venous blood using ethylenediamine tetraacetate potassium anticoagulation tube in training and quiet condition respectively, and then using flow cytometric to test the quantities of CD3~+,CD4~+,CD8~+ and the apoptosis condition of peripheral blood lymphocyte.Data processing methods: the data is calculated by FACS Calibur Cellquest 2.0 software. First, input the original data to excel software to store, and then transform it into DBF format. The data statistics is finished by SPSS11.5; the result data is showed by mean±sd and percentage; the total difference is analyzed with F. The relative analysis about AI and controlling factors uses torque Pearson correlation coefficients product. The statistical analysis of data comparison of each group before and after training uses Paired Samples T.Research resultsCD3~+ cell ratio of athletes after training is declined, especially large load training group, but drop weight group's is slightly increased.CD4~+cell ratio after training is obviously declined, and surpasses the quiet state level when dropping weight.CD8~+cell ratio is basically stable before or after training or during the period of dropping weight. CD4~+/CD8~+ ratio decreased after training, especially after large load training. It recovers to relatively quiet state level during the period of dropping weight.Early lymphocyte apoptosis of moderate load group is lower than relatively quiet group. However the middle-late apoptosis is obviously higher than relatively quiet group, with significant differences.After big load training, the peripheral blood lymphocyte apoptosis of free combat athletes is increased obviously. Among them, middle-late lymphocyte apoptosis is more obvious than the early period.During the period of dropping weight, the early blood lymphocyte apoptosis is decreased, but increased during the middle-late period.ConclusionsCombination of moderate exercise intensity, load and duration not only can effectively improve body function and immune function, but also can effectively improve body resistance. The immune system will change for good and there is no change for stress hormones which suppress the immune function, inflammatory and apronounced anti-inflammatory cytokines during moderate exercise. Moderate exercise is good for body immune monitoring function and can reduce the risk of infection.Large load training leads to the significant increase of peripheral blood lymphocyte apoptosis, especially in the middle-late period. Large load training results in the decrease of lymphocyte, the decline of immune function, and the causes the immune suppression. The increase of athletes'peripheral blood lymphocyte apoptosis may illustrate that apoptosis is one of the reasons why the number of lymphocytes caused by sports training decreases. Its mechanism could be launching apoptosis signal transduction pathways. Large load training in launching apoptosis signal transduction pathways could cause apoptotic; at the same time, it also causes immunosuppression, which may be a new sports training research field.