During the process of constantly improvement of market economy system and development of educational system, universities are facing unprecedented challenges and there have appeared stress and job burnout of university teachers in varying degrees. Therefore, the research is extremely important to promote healthy and harmonious development of university teachers.In order to discuss the relationship of stress, job burnout and mental health, job burnout is used as mediator variable in this paper. With PSS, SWLS, EESS, Emotion rating scale, SES and HADS, about 220 university teachers were investigated and studied, then, the data were analyzed by statistical treatment with SPSS 15.0. After Analysis of Univariate, Correlation Analysis and Path Analysis, the result showed: 1) In general, there have appeared the stress of medium or high degree and the job burnout of high degree while the degree of mental health level were lower than normal; 2) The stress may lead to job burnout, thus affecting mental health; 3) Job burnout is the important mediator factor which the stress affect their mental health.
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