The Effect Of Supplemented Ferrous Of Altitude Training On Some Irion Metabolism And Function Of Respiratory Chain In Mitochondria From Mice's Skeletal

The purpose of altitude training is planned to organize athletes to altitude areas with regular special training to enhance athletic performance. Synthesis of iron as hemoglobin, myoglobin and various enzymes of energy metabolism in one of the main raw material, involved in oxygen transport, storage and energy metabolism, exercise changes in iron metabolism in skeletal muscle cells will directly affect athletic performance. The body reserves of iron is the effect of altitude training is an important factor. In this study, which make use of hypoxic cabin equipment simulating simulated altitude training environment, building-up animal training methods. The low-pressure chamber using artificial simulated altitude environment (altitude 3500m), the establishment of different doses of altitude training compound added iron model, by measuring the hemoglobin in blood, iron metabolism-related indicators, altitude training course to explore the body changes of iron metabolism . Skeletal muscle by measuring the mitochondrial respiratory chain enzyme complexes NADH-COQ reductase (CI), succinate reductase (CII), COQ -cytochrome reductase (CIII) and cytochrome oxidase (CIV) activity of the plateau Training Complex iron supplement on body function of mitochondrial respiratory chain, and iron supplements for the altitude training on body iron metabolism and mitochondrial respiratory chain function of reference data.Experimental Methods:To healthy male Wister rats aged 2 months, experimental subjects, the treadmill and high altitude adaptation of the screening, will retain the 40 rats were randomly divided into 5 groups: Quiet group (C); exercise group (H); small doses iron exercise group (X); the dose of iron supplementation exercise group (Z); high-dose iron supplementation exercise group (D). Rats living in simulated altitude of 3500m altitude or high-intensity treadmill incremental training, fed supplement of ferrous sulfate. Increasing speed from 25m/min to 35m/min, exercise time increased from 30min to 60min. Training 1 day, 6 days a week training, trained a total of 5 weeks. The end of the fifth week , have a rest 24 hours in the plain , and one-time all rats were killed by decollation after exhaustive, the rats were measured hemoglobin, serum iron, total iron binding capacity, transferrin saturation and the activity of skeletal muscle mitochondrial enzyme complexⅠ,Ⅱ,Ⅲ,Ⅳ.Result of the experiment:①Hemoglobin: HB rats compared with the plain, on the plateau of various rat hemoglobin were significantly different. On the plateau, compared with C, H and Z increase hemoglobin group phenomenon, but not statistically significant (P> 0.05); X and D groups had significantly lower hemoglobin phenomenon (P <0.01). Compared with the H, Xand D were significantly lower (P <0.01); Z was no significant difference. Compared with the X, Z and D group was significantly increased (P <0.01, P <0.05). Compared with the Z, D were significantly lower (P <0.01).②Serum iron: comparison with C, H and Z are growth phenomena, but no significant difference; D were significantly higher (P <0.05); X were decreased, no significant difference. Compared with the H, X, Z, D no significant difference. Compared with the X, Z no significant difference; D were significantly higher (P <0.05). Compared with the Z, D group increased, but no significant difference.③Total iron binding capacity: with C, H group decreased the phenomenon, but no significant difference; X, Z, D decreased significantly (P <0.01, P <0.05). Compared with the H, X group reduced the phenomenon, but no significant difference; Z and D were significantly lower (P <0.05). Compared with the X, Z and D were decreased, there was no significant difference (P <0.05). Compared with the Z, D group decreased, there was no significant difference (P <0.05).④Transferrin saturation: comparison with C, H, X, Z, D group were significantly higher (P <0.01, P <0.05). Compared with the H, X group increased, there was no significant difference; Z and D were significantly higher (P <0.01). Compared with the X, Z and D were significantly higher (P <0.05). Compared with the Z, D group increased, there was no significant difference (P <0.05).⑤Mitochondrial respiratory chain enzyme complexesⅠ~Ⅳ: with the C group, H and X groups CⅠ~CⅣactivity were significantly increased (P <0.01); Z Group CⅠ~ CⅣactivity significantly increased (P <0.05, P <0.01); D group CⅠactivity was significantly decreased (P <0. 5), CⅡwas no significant difference (P> 0.05), but a certain trend of growth, CⅢ, CⅣactivity were significantly increased (P <0.05 , P <0.01). Compared with the H, X, Z and D group CⅠ~ CⅣwere significantly reduced activity (P <0.01). With the X group, Z group CⅡactivity decreased significantly (P <0.01), CⅠ, CⅢno significant reduction (P> 0.05), CⅣactivity was significantly increased (P <0.05). D Group CⅠ~ CⅣwere significantly reduced activity (P <0.01). Compared with the Z, D group CⅠ~CⅣwere significantly reduced activity (P <0.01).Conclusion:①Altitude training group and the hemoglobin content in the higher dose of iron supplementation group, and the dose of iron in the hemoglobin group increased most significantly.Added different doses of iron have different effects on the rat HB; content. Tip of altitude training capacity of the blood transport oxygen; and low hemoglobin may be due to abnormal metabolism of nutrients lead.②Altitude training group, altitude training compound in the dose and high dose iron supplementation increased serum iron, low dose iron supplementation significantly decreased serum iron.Altitude Training Complex iron group, the total iron binding capacity, transferrin saturation significantly increased phenomenon.Tips lead to iron overload iron dose of the General Assembly, and Exercise capacity; altitude training environment, strength of the size of the different doses of iron supplementation on iron metabolism have significant effect on the body.③Altitude training group significantly increased immediately after exhaustive rat skeletal muscle mitochondrial respiratory chain CⅠ~ CⅣactivity.Altitude Training Complex iron group may also improve skeletal muscle in rats immediately after exhaustive mitochondrial respiratory chain CⅠ~ CⅣactivity, and the altitude training combined with small doses of iron supplementation is better than altitude training complex in iron dose and high dose group, which simply the best altitude training group.Altitude training tips antioxidant activity in skeletal muscle mitochondria and respiratory chain complex enzyme activity an association to improve the skeletal muscle of exercise-induced fatigue, increased mitochondrial oxygen utilization capacity, and ultimately improve athletic performance.In summary, altitude training can improve the body composite iron groups iron storage capacity, when the exercise using the body iron reserves, the additional amount of trace elements of iron, use of prevention and treatment of iron deficiency caused by the body's ability to reduce movement.Amount of iron the body can increase the hemoglobin value is conducive to oxygen transport, thereby enhancing the body's energy supply.In the enhanced skeletal muscle mitochondrial respiratory chain function, the simple set of altitude training, altitude training combined with small dose, the doses were increased significantly, the simple effect of altitude training group of the most significant, suggesting a reasonable course of altitude training nutrition supplements, proper strengthexercise training can improve the body ability.