| Simulation altitude training is a kind of new scientific training methodsdeveloped in the several years and plays a positive role in sports training. Thisexperiment uses the low pressure of oxygen tank equipment simulation different lowoxygen training method and establishes the animal training model, using biologicalchemical theory and cell biology research methods to rat skeletal musclemitochondrial enzyme respiratory chain composite biological activity and free radicalcontent in omparison to seek the low oxygen environment of sports training the besttraining method.Test methodThe experimental object is male health Wister rats and the rats were sixty malehealth two months of Wister rat only sixty (provided by Gansu institute of traditionalChinese medicine laboratory animal center), weight one hundred±ten gram.Pointscage breeding, every cage ten only. The national standard feed rodents, free fooddrink, room temperature±two degree about twenty three, relative humidity fortypercent to sixty percent, the natural lighting. Feed room, such as appliance regulardisinfection.(1) Ptation: rats after buying in animal breeding room two days, making rats toadapt to the animal room environment.(2) Taiwan: all rats often oxygen conditions one time per day of two days oflevel run a adaptation, each exercise intensity from twelve meters per minute began toincrease to eighteen meters per minute, and sports time is fifteen minutes. Thefamiliar with run sets.(3) Ength adaptation: rats running back to one time per day for one week of thestrength of the adaptation, athletic intensity from twenty meters per minute began,increasing every day three meters every minute, achieve finally twenty five meters perminute and sports time is fifteen minutes. Make rats gradually adapted to formalendurance training intensity.(4) Itude adaptation: rats strength training every day, all in lab rats in oxygenoften live height is two thousand five hundred. (5) Imal screening: according to body weight, adaptive training, eliminatedunderweight or overweight, not adapt to the low oxygen environment and run amovement of the rats, keep sixty mouse for formal experiments.(6) Mal group will retain the rats randomly divided into five groups. Quiet group(A, n equal to ten); A week training group (B, n equal to ten), two weeks traininggroup (C, n equal to ten); Three weeks training group (D, n equal to ten); All roundtraining group (E, n equal to ten) five weeks training group (F n equal to ten).(7) Training program: According to the A.X.Bigard treadmill training scheme,low pressure oxygen cabin altitude2500m simulation plateau environment, the levelof animal running load training, rats five weeks of training, train six times a week,using HHL training mode. The hypoxic training. Hypoxia living normoxic training.Each training30minutes Monday to Saturday, which normally aerobic training, andthe weekly2.4.6plus a hypoxic training. Hypoxia and sleep for12hours. Hypoxiatraining and hypoxia during sleep oxygen partial pressure of15.4%, equivalent to theheight above sea level2500, atmospheric pressure, normoxic training in simulatedaltitude1500meters.The experimental results(1) Mparing to quiet A group, the exercise group BCDEF rats hemoglobincontent of A significant rise is less than zero point zero one.(2) Paring to quiete group A, mobile group BCDEF rats enzyme activity of theactive chain was significantly increased tendency, in which the activity of enzyme Ichange the most activeis less than zero point zero five.(3) Paring to quiet A group, the exercise group BCDEF rat ATP enzyme activityis rising and is less than zero point zero four.(4) Paring to quie A t group, the exercise group of BCDEF rats number of whiteblood cells A rising trend is less than zero point zero one.Conclusion1HiHiLo training to improve rat hemoglobin content of first to fourth weeksshowed an upward trend, aerobic capacity, the rat red blood cell number in the threeweeks before the change is obvious rise. According to the content of hemoglobin andred blood cell changes in the number and work out a reasonable training time, visible movement training is the effective means to maintain the hemoglobin content.2HiHiLo training can significantly improve the rat heart mitochondrialrespiratory chain C I~C IV activity, which in the two or three week of enzymeactivity changes most apparent, that is to say in the first three weeks to achieve thebest effect.3HiHiLo training can enhance rat ATP enzyme activity changes, in first weeksis not obvious, but the2.3.4weeks after changes showed significant changes, increasedobviously, he takes three weeks to achieve the best results. |