| Mitochondria are the places provide energy movement in the mitochondria, within the way by oxidative phosphorylation of the energy stored energy material for chemical conversion (ATP), in order to meet the needs of the body. This study used to rat exogenous added ferrous sulfate with sports training for experimental model, using the physiology and biochemistry of the theory and method of rats, skeletal muscle enzyme activity and mitochondrial respiratory chain radicals metabolism indicators analyzed statistical research through the sports training and supplement of ferrous sulfate rats to the respiratory chain enzymes and free radicals metabolism, through the study, hope for the workers provide relevant theoretical basis.Method of experimental and object:Five weeks Wistar rats 60 healthy male, weight, adaptability is 100 gold in two angels to feed animals ran machine room environment and the animals. Ran machine adaptive method: every day running platform for 0 degrees slope, ran machine speed increasing from 10m/min 12m/min, time to run for 10min Taiwan training. Experimental animals shall be divided into: quiet group (C, n = 10), in the exercise group (T,n = 10), small doses of iron in the exercise group (S,n =10 ), and in iron, and in the exercise group (M,n = 10), large doses of iron in the exercise group (L, n = 10). All week weigh weight a rat day, iron supplements by way of ferrous sulfate gastric lavage solution. The dose of iron:Small doses of iron group every day 28.85 mg/kg mouse and rat weight in each rat day iron group by 57.7 mg/kg weight, large doses of rats iron group 115.4 m/kg,g per day by rat rat/kg weight. In the exercise group and iron + movement rats were increasing load ran machine training 8 weeks, six days per week training and rest on Sunday, the animal ran machine for 0 degrees slope.4 weeks before the exercise-induced anemia model is established for the training plan: two weeks before 1st day training, the rest of the time for each 1 times every morning and evening, For the first time after 2 min / 5min, the acceleration of times, and finally 1 times for increasing the training time for 101 min, After the training ZhouTie supplement plan for: twice a day (twice), the same time training for two weeks before the 1980s min/times and 85 min/times, after two weeks in 90min/times as a starting point, 2 min/times for increasing the acceleration. Article 8 weeks after training on Saturday 24 hours, will all rats running through the disposable anesthesia machine movement to exhaustion after death. Rats were determined, the exhaustion of time, skeletal muscle weight weight index, hemoglobin content, serum iron content, mitochondrial enzyme complexesâ… -â…£and the content of MDA and SOD, GSH - PX activity index.Experimental results:1.Compared with the group C, S,M,L prolonged exhaustion group (P < 0.01), and T groups S group of rats and M group burnout time with significant difference (P < 0.05).2.Compared with the control group, quiet, in small doses, dosage, large doses of iron and sports rats were increased Câ… activity (P < 0.001), T,S,M. group Câ…¡activity increased (P < 0.001), T, S, M and L rats, Câ…¢activity increased (P < 0.001), t., s., L rats, C, Mâ…£activity rats, Câ…£activities (P < 0.001). Compared with the sport group, S, L rats, Câ… activity (P < 0.001), M group Câ… activity increased (P < 0.001), S, L group Câ…¡activity (P < 0.001), M rats, Câ…¡activity increased (P < 0.001), S, M rats, Câ…¢activity increased (P < 0.001), S, L rats, Câ…£activity (P < 0.001), M group Câ…£activities (P < 0.001).3.Compared with the control group, the content of MDA motion reducing (P < 0.01), iron in plus exercise the MDA content increased (P < 0.001), T rats SOD increased (P < 0.05), s. m., SOD activity of rats, (P < 0.001), L rats SOD reduce (P < 0.05). S, M rats, GSH - PX activity increased (P < 0.001), L rats, GSH - PX activity (P < 0.001). Compared with the sport group, s. m., MDA content in rats, increased (P < 0.001), L rats, MDA content was increased (P < 0.05), S, M rats SOD increased (P < 0.001), L rats SOD reduce (P < 0.001). S, M rats, GSH - PX activity increased (P < 0.001), L rats, GSH - PX activity (P < 0.001). The experimental results:1.Rats running in Taiwan to exhaustion once in a relatively short time, long-term endurance sports training and supplement of ferrous sulfate could significantly prolong rats running platform to exhaustion once time, improve the body's ability to exercise.2.Added ferrous sulfate plus exercise training can make rats skeletal mitochondrial enzyme Câ… - Câ…¢activity to improve, but the activity of Câ…£. Through the complement of ferrous sulfate could accelerate oxidative phosphorylation process, and provide favorable conditions for the body to synthesize ATP, improve the level of function.3.Through the complement of ferrous sulfate plus exercise training is the content of MDA skeletal rats, mitochondria, GSH-PX,SOD activity. |
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