| As the component of electron transport and proton transference of mitochondrial respiratory chain,ferrous sulfate regulates the physical function of mitochondrion, tranferring protons among respiratory chain complex I, II, and III, and functioning in synthesis of ATP. According to the extant researches, ferrous sulfate is involved in the speed of ferrous sulfate mitochondrion proton transferrence. This research investigates the effects of ferrous sulfate supplement on sport-training, based on the observation of activiy of CI~CIV of respiratory chain complex in liver mitochondrion. This research is meant to seek ways of improving hepar mitochondrion respiration by proper nutrition supply.Methodology description:Choose 60 healthy male Whister rats with average weight of 100 g(proximately, 5-week old), raise the rats for 2 days, and then devide them randomly into control group (C, n=10), training group with small amount of ferrum supply (S, n=10), training group with middle amount of ferrum supply (M, n=10), training group with large amount of ferrum supply (L, n=10) and training group (T, n=10). Arrange 8-week's treadmill training of increasing endurance for the training group and ferrous-sulfate-supply plus trainning group, but not for the control group.The training is devided into two stages:At the first stage all groups except the control group do training without any medicine application to get (IDA) model rats. 4 weeks later (IDA) model rats are prepared.At the second stage, all rats except the rats in the control group do training and supply ferrous sulfate of different amounts to rats but not to the rats in the simple-training group. Supply ferrous sulfate to the rats in the training plus medicine-supply group between 7:00 pm-8:00pm, and suuply distilled water to the control and the training groups. In the 8th week late, all rats do treadmill training until they get exhausted; kill the rats immediately; test the SOD activity, GSH-Px content, MDA content, and activiy of CI~CIV of respiratory chain complex in hepar mitochondrion in the rat's body.Results (1) Compared with the control group, the activity of SOD of the rats in small-amount and middle-amount ferrum-supply groups shows significant difference (P<0.01), and the amount of MDA in the rats increases significantly (P<0.05) when the rats are exhausted in treadmill running. Compared with the training group, the activity of SOD of the rats in small-amount ferrum-supply and training groups shows significant increase. The amount of MDA in the rats increases significantly (P<0.05) when the rats are exhausted in treadmill running. Compared with the non-training group, the GSH-Px in rats of middle-amount and large-amount ferrum-supply groups shows significant difference. Small-amount ferrum-supply group shows no significant difference compared with the training group. Middle-amount and large-amount ferrum-supply groups show no significant difference compared with the small-amount ferrum-supply group.(2) Compared with the control group, the activity of the hepatic mitochondrial CI, CII, CIII of the rats in ferrous sulphate supply group increases significantly (P<0.01), and the activity of CIV of the rats changes obviously (P<0.05). The activity of CIV, C11 of the rats in the training group increases significantly (P<0.05).Conclusion(1) Ferrous sulphate supply doesn't significantly affect Fe content in the rat's body; ferrous sulphate supply significantly affects GSH-Px content in the rat's body.(2) Ferrous sulphate supply of small amount significantly increases the activity of SOD in the rat's boady, and it also increases MDA content in the rat's hepar. Training plus ferrous sulphate supply of middle and large amount significantly increases GSH-Px in the rat's body (P<0.001).(3) Ferrous sulphate supply significantly increases the activity of the enzyme CI and CIV of the hepatic mitochondrial respiratory chain, positively affects the activity of CIII and CIV, and prompts the process of oxidation and phosphorylation of mitochondrial, helping mitochondrial synthesize ATP. However, ferrous sulphate supply doesn't significantly delay training-induced fatigue. |
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