Investigation On The Expression Of Mu-Opioid Receptors And The Visual Modeling Of Its Distribution In Adult Brain

Objective:The expression of the mu-opioid receptors in adult brain has been studied by using tissue microarray and immunohistochemical technique. Some other works, which include the mu-opioid receptors comparison between the regions in adult brain to see whether the differences has statistically significant or not and analysis of the relationship between the distribution of mu-opioid receptors and anatomical structure, have also been completed in this paper. Furthermore, the three-dimensional perspective has been established by making use of the method of structural reconstruction in computer three-dimensional reconstruction graphics, and the intuitional mu-opioid receptors distribution model has been obtained. It's provided experimental support for researching the mechanism of opioid dependence formation and the withdrawal of opioid addiction.Methods:The brain specimens used in this paper which have completely structure and no visible lesions in autopsy are collected, and the entire brain are cut into small pieces(5mm*5mm*5mm) by using position select instrument, then the coordinates of each pieces are taken for the record. After making these pieces into normal paraffin blocks, the Tissue microarray is established and sliced. The slices are stained by making use of immunohistochemical technique and rabbit anti-human monoclonal antibody of mu-opioid receptors, and the expression of mu-opioid receptors and the distribution are detected. At last, the software SPSS is applied in this paper for statistical analysis of the data which is obtained by detection. The software 3D surfer is also taken to complete the 3D reconstruction for the distribution of mu-opioid receptors, and the visualized 3D model of the distribution of mu-opioid receptors is acquired. Results:The expression of mu-opioid receptors in various parts of the cortex is compared under the circumstances of P>0.05, and the results show that the difference of expression intensity has no statistically significant. The expression of mu-opioid receptors in various parts of the brain is compared under the circumstances of P<0.05, and the results show that the expression intensity is not exactly identical. Furthermore, under the circumstances of P<0.05, the pairwise comparison of all parts of brain is also executed each other, and the results show that the part of most intense expression of mu-opioid receptors in brain is pons, and then is striatum, midbrain, thalamus, cortex, and cerebellum. the pairwise comparison is also done for the identical region of brain between male and female under the circumstances of P>0.05, the results show that the difference of expression intensity has no statistically significant.Conclusions:1. The expression of mu-opioid receptors in various regions of brain is different, the part of most intense expression of mu-opioid receptors in brain is pons, and then is striatum, midbrain, thalamus, cortex, and cerebellum; there is no expression in white matter;2. The difference of expression intensity between male and female for the mu-opioid receptors in brain has no statistically significant;3. The visual modeling of mu-opioid receptors is benefit to research in the mechanism of opioid dependence formation by displaying the experiment results intuitively.