The Effects Of Needle–Embedding Therapy In Dingchuan Point To EOTAXIN Protein And Its MRNA Exprexxion Of Asthma Model Rats

Purpose:In order to observe the effets of method with needle–embedding therapy in Dingchuan point to the asthma rat modelEotaxin protein content and their mRNA, inquiry into the function mechanisms of with needle-embedding therapy in Dingchuan point to bronchial asthma ,provide new treatment methods and theoretical basis for clinical treatment.Methods:Selecting healthy 4-6 week-old Wistar rats were 40as targets in this experiment,which were randomly divideded into groups. Sensitized with OVA to stimulate the rats to establish the asthma model. 40 Wistar rats were randomly divideded into 5 groups, control group(8),asthma rat model group (8),dexamethason group (8),needle– embedding therapy group(8) and acupoint injection group (8).Observe the pathological changes in lung tissues with light microscope.Adopting immunohistochemistry method and hybrid diz -ation in situ method to examine the expression of asthma rat models of Eotaxin protein and their mRNA。Results:1 After the success of modeling, needle-embedding therapy group, dexamethasone group and acupoint injection group, compared with the asthma model group, significantly reduced the symptoms in rats,latent period of asthma was significantly prolonged, falls rate was significantly reduced. Statistically, there are significant differences in them(P<0.01), needle–embe -dding therapy group and dexamethasone group are similar, there was no significant difference between them(P>0.05).2 The expression of Eotaxin protein and their mRNA were significantly higher than the normal control group in asthmatic rat model group(P<0.01). Eotaxin protein contents were lower than the model group in needle-embedding therapy group, dexamethasone group and acupoint injection group(P<0.01), theirs mRNA expression was significantly weaker than the model group, Statistically, there were significant differences in them (P<0.01). Needle-embedding therapy group and dexamethasone group were similar, there was no significant difference between them(P>0.05).Conclusions:1 Needle-embedding therapy in Dingchuan point can signify -cantly reduce the level of rats with symptoms of asthma attack.2 By reducing the contents of the Eotaxin protein in the rat models of asthma,downing the expression of the EotaxinmRNA in airway epithelial cells,reducing the levels of BALF so as to achieve the purpose of treatment of bronchial asthma.