Experimental Study Of Effects Of "Qingrejiedu And Liangxuetongluo" Recipe In Treating Mspgn Rats

Objective: On the basis of previous experiments, observe the effect of"QingreJiedu and LiangxueTongluo"Recipe in treating mesangial proliferative glomerulonephritis (MsPGN) rats on serum creatinine (SCr), serum albumin (ALB), glomerular mesangial cell (GMC) and extracelluar matrix (ECM), and test the expression of nuclear factor-kappaB (NF-κB), B-cell lymphoma-2 gene (Bcl-2) and tumor necrosis factor-α(TNF-α) in glomerular mesangial area, to explore the mechanism of"QingreJiedu and LiangxueTongluo"Recipe.Methods: Male SD rats were adopted as the study objects in the experiment. Replicated the MsPGN rats model by taking orally and time-controlled vein injecting boving serum albumin (BSA), vein injecting staphylococcus enterotoxin B (SEB), spot of division of hypodermic injecting complete Freund's adjuvant and not complete Freund's adjuvant. Divided 32 rats into blank group, model group, therapy group ("QingreJiedu and LiangxueTongluo"Recipe group) and comparison group (Tripterygium Glycosides group). Observed the effect of"QingreJiedu and LiangxueTongluo"Recipe and Tripterygium Glycosides (TPG) as a comparison for the rat model with MsPGN on renal function. Measured level of SCr, serum ALB in blood of different periods, observed the proliferation of GMC and ECM by light microscope, measured the expression of NF-κB, Bcl-2 and TNF-αof nephridial tissue by immunohistochemical method and took quantitative analysis.Results: 1. There were more SCr in blood (P<0.05), but lesser serum ALB in model group than those in blank group (P<0.01), light to moderate mesangial proliferative pathological, which demonstrated the mesangial proliferative nephritis model was successfully duplicated. 2. Both therapy group and TPG group had increased serum ALB since 6th week(P<0.05), but there wasn't obvious difference between the two (P>0.05). 3. Both therapy group and TPG group had decreased SCr in blood since 8th week (P<0.05), but there wasn't obvious difference between them (P>0.05). 4."QingreJiedu and LiangxueTongluo"Recipe and TPG obviously alleviated proliferation of GMC and accumulation of ECM (P<0.01). 5. Compared with model group, therapy group and comparison group could visibly decrease the NF-κB, Bcl-2 and TNF-αconcentration (P<0.01). According to the result, therapy group and comparison group played important roles in the treatment, but there was no significant difference between them (P>0.05). 6. Results of renal pathology of MsPGN rats suggested that there were significant positive correlations among NF-κB, Bcl-2 and TNF-αin renal tissue (γ>0.5, P<0.01), which prompted the increase of quantity of NF-κB in renal tissue might lead to the increased expression of both Bcl-2 and TNF-α. 7. The hyperplasia intensity of glomerulus mesangial area correlated with power of the expression of NF-κB, Bcl-2 and TNF-α. Both"QingreJiedu and LiangxueTongluo"Recipe and TPG might alleviate renal pathology damage by restraining activation of NF-κB, reducing the expression of Bcl-2 and TNF-αin renal tissue, inhibiting the proliferation of GMC and accumuliation of ECM, as well as increasing serum ALB and decreasing SCr in blood.Conclusion: 1."QingreJiedu and LiangxueTongluo"Recipe can prevent the reduction of SCr, and inhibite the rise of serum ALB. 2."QingreJiedu and LiangxueTongluo"Recipe can inhibite GMC proliferation and ECM accumulation. 3. Our study indicates that"QingreJiedu and LiangxueTongluo"Recipe can alleviate the proliferation of GMC and accumuliation of ECM by inhibiting the expression of NF-κB, Bcl-2 and TNF-αof nephridial tissue, whose effect is considerable with TPG. 4."QingreJiedu and LiangxueTongluo"Recipe can alleviate the pathological injury from different links, which reflects the therapeutic advantage of the traditional Chinese medicine compound multi-target, has a good clinical prospects.