| Objective:To observe the effect of Fushen Jiangzhuo decoction(FJD) on the Biochemical indicators quantitative, and kidney tissue morphology, and the expression of fibrotic factors in mesangial proliferative glomerulonephtitis(MsPGN) rats.The aim is to study the possible protecting mechanisms of FJD on renal fibrosis rats. Methods:1. 24 healthy Wistar rats were randomly divided into four groups: normal group, model group, FJD group, benazepril group. We gave them normal diet after adaptive feeding week. To reconstruct MsPGN model and extend to 12 weeks in order to cause developed renal fibrosis in rats.2. From the the first day of the experiment until the end of the experiment, treatment groups were given medicine of FJD and benazepril separately, FJD group was gavaged with a dose of 51.5g·kg-1·d-1 FJD, benazepril group was gavaged with a dose of 4mg·kg-1·d-1. The two medicines were administered by the volume of 10ml·kg-1.3. 2 days before the 12 weekend, we collected 24-hour urine of four group rats with metabolic cages to test quantity of 24-hour urinary protein; at the 12 weekend, the blood was taken from abdominal aorta to detect BUN, SCr and β2-MG level in the rats.4. At the 12 weekend, the rats were sacrificed. The abdomen of the rats was opened quickly and the kidneys were taken out, and then divided into several parts of different sizes for HE, Masson stainin and immunohistochemistry assay. Then the pathological changes of renal tissue were observed.5. The protein expressions of TGF-β1, Smad3, ILK, ColⅠ, BMP-7 and HGF in renal tissues were detected respectively by immunohistochemistry assay. We can explore the effect of FJD on TGF-β1-Smads-ILK signaling channel and fibrosis factors.6. The experimental data of every group was compared with SPSS17.0 statistical software. Based on homogeneity of variance results, single factor analysis of variance and non-parametric test were used to understand the relationship between each indicator. Finally, we analyse FJD on relevant indicators and preliminary explore the mechanism of its inhibition of renal interstitial fibrosis. Result:1. Compared with the normal group, 24 h urine protein, BUN, SCr, β2-MG of model group was increased significantly(P<0.01), FJD and benazepril group were decreased significantly compared the model group(P<0.05).2. The results of light microscopy showed renal tubular epithelial cell necrosis, mesangial proliferation and interstitial fibrosis significantly in the model group; the damage degree was alleviated in FJD group and benazepril group(P<0.01).3. Immunohistochemistry results showed that: TGF-β1, Smad3, ILK, ColⅠprotein expression level of the model group were significantly higher than the normal group(P<0.01), but they were significantly decreased in FJD group and benazepril group(P<0.05).The protein expression of BMP-7 and HGF in Model group were significantly decreased than normal group(P<0.01), but they were significantly increased in FJD group and benazepril group(P<0.01). Conclusion:The results showed that: FJD had a good protective effect on renal fibrosis in MsPGN rats; the effect mechanism of FJD may be related to reduce the levels of 24 h urinary protein, BUN, SCr and β2-MG and then to improve renal function. FJD can inhibit the protein expression of fibrosis factors including TGF-β1, Smad3, ILK, ColⅠ, and promote secretion of anti-fibrotic factors BMP-7, HGF. FJD could block TGF-β1-Smads-ILK signal transduction to relieve renal interstitial fibrosis. |
