Study On The Relationship Between Matrix Metalloproteinases - 9 (MMP-9) Gene Polymorphism And Recurrent Spontaneous Abortions

Objective:By determining the normal pregnant women and recurrent spontaneous abortions history woman's matrix metalloproteinases - 9 (MMP-9) gene polymorphism of peripheral blond to probe the relativity between MMP-9 gene polymorphism and recurrent spontaneous abortions.Methods:Using polymerase chain reaction - restriction fragment lengthpolymorphism (PCR - RFLP) analysis method to analyse sixty cases patients who have recurrent abortions history (case group) and 60 cases of normal health early pregnantwomen's (control group) MMP-9 gene polymorphism , Using t test to checkout the difference of age, gestational weeks between the case group and the control group. Comparing two groups gene frequencies and allele frequencise uses the chi-square test(χ~2 ) analysis. Calculate odds ratio(OR ) to show relative risk degree,and its 95% confidence interal (CI) .Results:(1) The case group and control group CT + TT gene frequencies are 10% and 25% respectively,two groups'comparison (χ~2=4.675,P﹤0.05),the difference have statistically significant.(2) The case group and control group T allele frequencise are 12.5% and 5%, respectively, two groups'comparison(χ~2=5.483,P﹤0.05) the difference have statistically significant.(3)Compared with CC genotype and C alleles, carrying CT + TT genotype and T alleles crowd can increase the risk of recurrent miscarriage, value at risk (95% CI = 3.000 1.075-8.370), 2.714 (95% CI = 1.015-7.255) respectively.Conclusion:(1) MMP-9 gene polymorphism in serum may be the risk factors which cause recurrent spontaneous abortions .(2) T allele may be recurrent spontaneous abortions genetic susceptibility genes.