| Objective:1.To analyze the relationship between MTHFRC677T gene polymorphism,homocysteine(Hcy)and recurrent abortion;2.To investigate the necessity of detecting MTHFRC677T gene polymorphism and homocysteine in pregnant women for the diagnosis of recurrent abortion;3.According to the test results,appropriate folic acid supplementation should be given to pregnant women as needed to improve the pregnancy outcome of patients with recurrent abortion.Methods:A total of 60 pregnant women were selected from the outpatient department and inpatient department of the department of obstetrics and gynecology of the affiliated hospital of Inner Mongolia medical university from December2017 to January 2019.In the control group,30 women with no spontaneous abortion and the same gestational age as those with spontaneous abortion were randomly selected to visit the hospital at the same time and had at least one successful pregnancy.Peripheral venous blood was extracted to detect MTHFRC677T gene polymorphism and plasma Hcy value,and the relationship between recurrent abortion,MTHFRC677T gene polymorphism and plasma Hcy was studied by statistical methods.Results:(1)The age range of patients in the case group was23~39 years old,the number of pregnancies was 2~5,the BMI was 19.05~30.84Kg/m2,and the height was 158cm~170cm.It was calculated that the average age of patients in the case group was 30.10±4.93 years old,the average height was 162.47±2.97cm,and the average number of pregnancies was 2.50(1.00).The average BMI was 23.35±2.43kg/m2.The age range of the control group was 22~40 years old,the number of pregnancies was 1~6,the height was 155cm~171cm,and the body mass index was 20.42~31.25Kg/m2.The average age of the control group was 30.50±5.30 years old,the average height was 162.67±4.63cm,the average number of pregnancies was 2.00(1.00),and the average BMI was 24.42±2.52Kg/m2.There was no significant difference in age,height,weight,pregnancy and body mass index between the two groups(P>0.05);(2)In the control group and the group of patients in case group TT genotype frequency was 26.70%(8/30),computed tomography(CT)genotype frequency was 53.30%(16/30),the CC genotype frequency was 20.00%(6/30),control group TT genotype frequency was 16.700%(5/30),computed tomography(CT)genotype frequency was 50.00%(15/30),the CC genotype frequency was 33.30%(10/30),MTHFRCC,CT,TT genotype distribution of different frequencies,there was no statistically significant difference(X~2=1.725,P=0.422>0.05).The C allele frequency was 46.70%(28/60)in the case group,53.3%(32/60)in the T allele frequency,58.30%(35/60)in the control group,and 41.70%(25/60)in the T allele frequency.The difference in allele frequency between the two groups was not statistically significant(P>0.05);(3)The results of plasma Hcy in the case group and the control group were different.Among them,the average plasma Hcy value in the case group was 13.98±2.05umol/L,significantly higher than the average plasma Hcy value of 11.95±1.36umol/L in the control group.The difference between them was statistically significant(t=4.527,P<0.01);(4)In the case group,when the genotype was CC,the average plasma Hcy value was 13.35±0.64a umol/L.When the genotype was CT,the mean plasma Hcy value was 12.90±1.61a umol/L;when the genotype was TT,the mean plasma Hcy value was 16.62±0.77umol/L.In the control group,when the genotype was CC,the average plasma Hcy value was 10.40±0.58ab umol/L.When the genotype was CT,the mean plasma Hcy value was 12.34±0.56aumol/L;when the genotype was TT,the mean plasma Hcy value was 13.89±0.49umol/L;whether in the case group or the control group,the Hcy value of TT genotype was higher than that of both CT genotype and CC genotype.Conclusion:(1)In this study,there was no correlation between MTHFRC677T gene mutation and the risk of recurrent abortion;(2)The increase of homocysteine is correlated with the occurrence of recurrent abortion Increased risk of recurrent miscarriage;(3)The homozygous mutation of MTHFR677TT is one of the factors causing the increase of plasma Hcy level. |
PDF Full Text Request