The Study Of Ulcerative Colitis And TCM Spleen-stomach Damp-heat Syndrome With Intestinal Microecology

Ulcerative colitis (ulcerative colitis, uc) is an inflammatory bowel disease. So far, the cause of ulcerative colitis and pathogenesis is not yet clear. Multiplied in recent years, incidence increased year by year, so the disease is also more and more attention. Clinical study found that the incidence of intestinal flora is the UC is an important motivating factor, and in TCM, Spleen-stomach Damp-heat Syndrome and spleen deficiency syndrome and more common.1. Literature Research1.1 The modern medicine research on UCUlcerative colitis (ulcerative colitis, uc) is an inflammatory bowel disease. So far, studies that UC may be mainly decided by the genetic predisposition, immune disorders is a key mechanism for the direct incidence of intestinal flora is important that stimulate the immune injury factors, environmental factors such as mental disease may be an inducement. The pathogenesis of this series, finally led to the colon mucosa and submucosa damage, mostly involved the rectum and distal colon. Clinically, as countries have different diagnostic criteria, the current diagnostic criteria for internal comparison is recognized nationwide in 1993, Taiyuan, chronic non-infectious intestinal diseases symposium to discuss the newly revised standard (Taiyuan standard). For the treatment of patients diagnosed, in addition to medical treatment, nutrition, support, psychological and symptomatic treatment of adjuvant therapy is also very important.1.2 TCM studies on UCMany scholars believe that this disease is based on spleen and stomach Deficiency, Dampness partial victory. Onset of the disease caused by the incentive is improper diet, stomach injury, or emotional disorders, liver the spleen, resulting in transport of weak spleen and stomach, food stagnation wet win. For UC's Syndrome there is a consensus-based, according to 2003 China Association of Integrative Medicine Professional Committee issued digestive diseases ulcerative colitis diagnosis and treatment of Integrative Medicine (draft), the TCM Syndrome Differentiation of UC were divided into:①Spleen-stomach Damp-heat Syndrome;②stomach qi deficiency;③spleen deficiency syndrome;④stagnation spleen deficiency;⑤blood deficiency permits;⑥intestinal stasis network card. For various syndromes, there is a corresponding treatment. In clinical found to Spleen-stomach Damp-heat Syndrome and stomach qi deficiency in the majority.1.3 TCM and MicroecolocyChinese medicine believes that the main receiving the stomach, spleen governing transportation, it will be refined to transfer the body of material. Temper Jian Wan, the body organs to get adequate nutrition organizations, whereas spleen lose health movement, the body's digestion and absorption will be abnormal, leading to increased frequency of bowel movements and diarrhea leakage and other symptoms. And the normal human gastrointestinal microbial, carbohydrates, proteins, lipids play an important role in absorption. Such as gastrointestinal microbial structure and function of normal, will play the normal function of digestion and absorption. If the structure and function abnormalities, such as the reduction of bifidobacteria when the biological barrier function is conducive to the invasion of foreign pathogens, can cause diarrhea, flatulence and other performance. This loss of health and medicine the spleen is very similar transport.Modern bacteria through the gastrointestinal tract and diarrhea-predominant irritable bowel syndrome Spleen, Spleen chronic diarrhea and other research and found that stomach intestinal flora and the Chinese very closely.1.4 TaqMan quantitative PCRGiven the shortage of traditional bacterial culture method, TaqMan quantitative PCR technique is based on microbial genome DNA sequence information is based on analysis of environmental samples by type and quantity of DNA molecules to reaction microbial flora composition and community structure. This technical specificity, high sensitivity, good linear relationship, simple operation, high automation and pollution prevention. Used to determine the types and quantity of microorganisms may be of a higher degree.2. Experimental Study2.1 Objective:To investigate ulcerative colitis Spleen-stomach Damp-heat Syndrome relationship with the gut microflora2.2 Methods:The subjects were 24 cases of ulcerative colitis patients, the spleen and stomach in Chinese medicine was wet 17 cases of heat syndrome, spleen deficiency syndrome and 7 cases, another 10 normal subjects as control group. Fluorescent quantitative PCR using TaqMan technology, the study stool samples representative of the most important bacteria of intestinal flora-Bifidobacterium and Escherichia coli DNA fluorescent quantitative comparison between the stool specimens of each group the corresponding changes of microbial detection index and the UC major clinical symptoms and the relationship between tongue.2.3 Results:UC patient group content of fecal bifidobacteria, E. coli, B/E value and normal.One group did not differ (P> 0.05), while Bifidobacterium and there is no correlation between E. coli; according to UC distinction between clinical types and disease stages, intestinal flora, the early onset and chronic relapsing UC UC patients with fecal bifidobacteria levels, E. coli, B/E no difference between the values (P> 0.05). UC patients with early onset compared with normal, fecal bifidobacteria content and B/E values lower than those in group (P<0.05), E. coli no difference. Chronic relapsing UC patients and healthy controls group, fecal bifidobacteria levels, E. coli, B/E no difference between the values (P> 0.05). Active UC and UC patients in remission fecal bifidobacteria levels, E. coli, B/E no difference between the values (P> 0.05), active UC patients compared with normal subjects, fecal Bifidobacterium bacteria levels lower than those in group (P<0.05), E. coli and the B/E value of no difference. UC patients in remission compared with normal subjects, fecal bifidobacteria levels, E. coli, B/E no difference between the values (P> 0.05). UC patient group to further test the main clinical symptoms of intestinal flora and the link (diarrhea, abdominal pain, blood and pus, tenesmus, fever, abdominal distension, eating less, anal burning, soak in red, physical fatigue and Shenpi lazy Introduction), patients hot anal symptoms and faecal samples, the levels of Bifidobacterium significant negative correlation (r=-0.405, P=0.050), with E. coli and B /E value of no significant relationship. The main symptoms are related to the remaining content of Bifidobacterium, E. coli and B/E value of no significant relationship.Patients with ulcerative colitis Spleen stool specimens tested, Spleen, compared with spleen deficiency syndrome, fecal bifidobacteria were lower than spleen deficiency syndrome (P<0.05), E. coli and B/E value spleen deficiency syndrome and no difference. Spleen Compared with the normal group, fecal bifidobacteria levels lower than normal (P <0.05), E. coli and B/E value of no difference with normal group certificate. Compared with the normal spleen deficiency syndrome, fecal bifidobacteria, E. coli and B/E value of no difference between normal group. Spleen and spleen deficiency syndrome and stool specimens of Bifidobacterium and E. coli, but no significant relationship. Further test the main clinical symptoms of intestinal flora and the link (diarrhea, abdominal pain, blood and pus, tenesmus, fever, abdominal distension, food less, anal burning, soak in red, physical fatigue and Shenpi lazy Introduction), Spleen patients faecal samples, bifidobacteria, E. coli and B/E value and the main symptoms no statistically significant relation between. Intestinal flora and tongue observation links Spleen stool specimens Bifidobacterium, E. coli and B/E value of their tongue, tongue, tongue there was no significant relationship between the scope.2.4 Conclusions:2.4.1 Bifidobacterium and B/E decreased, and will weaken the Intestinal mucosa of various risk factors for the defensive role. Study authors found that patients with early onset UC fecal bifidobacteria content and B/E value lower than that group, active UC in patients with fecal bifidobacteria levels were lower than those in Group UC's disease and intestinal Tips Road-related microbial changes, and changes in intestinal flora of the pathological changes in the extent and severity of UC has some relevance. Degree of the patients with anal burning to reduce the content of Bifidobacterium.2.4.2 There is no UC Spleen of ecological studies, the author believes that Spleen fecal bifidobacteria were lower than spleen deficiency syndrome and normal group, and spleen deficiency same group and normal group, suggesting that UC may be hot and humid Card is in Xiesheng state, if the assumptions in addition to E. coli, there are other intestinal flora are also involved in the pathogenesis of UC, the upright body was suppressed by foreign evil, and so on behalf of righteousness of bifidobacteria decline, unable to resist foreign bacteria, causing intestinal flora, and then a series of clinical symptoms. The UC and normal intestinal flora of Spleen was no significant difference could be due to spleen and head of the CIA makes the increase in the number of Bifidobacteria.2.4.3 For future, I think we can focus with several aspects:①researchers must collect in case of an extended process, as far as possible to collect untreated patients with chronic persistent and acute outbreak of type UC is associated with the occurrence of intestinal flora.②the severity of the clinical treatment for UC has important reference value, while the severity of the present study failed to analyze whether the intestinal flora related, due to be involved in testing blood, ESR, and pathology, if research license against a disease severity to be a contact with the gut microflora.③Most previous studies are UC's traditional culture microbial detection, quantitative PCR with the experiment is different from the findings of this study, descendants still use the same experimental methods for validation.④There are many intestinal bacteria, and their UC and UC with the relationship between damp, to be further studied.⑤further heat Spleen Dampness and microbial disorders of Chinese medicine on the mechanism of beneficial therapeutic effects of Chinese medicine.