The Experimental Study That The Expressions Of Inflammator From Rabbits' Synovial Cells That Were Influ-enceed By Tongfengtai Granulars And The Clinical Research

ObjectiveThrough the experimental study that the tongfengtai granulars influenced the rabbits's synovial cells to IL-1β,IL-8,TNF-αand the ICAM-1 concent-rations, as well as through the observation of the clinical curative effects that tongfengtai granulars treated acute uarthritis arthritis patients, These experiments confirmed that the tongfengtai granulars had the strong anti-inflammatory and analgesia's function, also had the functions that cound prevented early time kidney harm.Experimental studyThere were three New Zealand rabbits which were lavaged respectively physiological saline,the tongfengtai granulars solutions and the diclofenac sodium capsule solutions, after one hour, collected centrifugal venou'serum from three New Zealand rabbits and named respectively numbers for serum 1, serum 2 and serum 3; we cultivated rabbits'knee synovial cells to 3 or 4 generations through the improved anization cultivate method and randomly divided into three groups. For example, blank groups that mixed into physiology-ical saline and seruml, model groups that mixed into urate and seruml, Chinese traditional medicine groups that mixed into urate and serum2, western medicine groups that mixed into urate and serum3; trained these synovial cells three days, determinaed contents of each group of synovial cells about IL-1β,IL-8,TNF-α,ICAM-1 through ELISA. Results:the model groups'contents about IL-1β,IL-8,TNF-α,ICAM-1 were highest than other groups obviously (P<0.05), but the outcomes about blank groups compare with the Western medicine groups or the Chinese traditional medicine groups'contents of IL-1β,IL-8,TNF-α,ICAM-1 were no statistical significance (P>0.05). Conclusion:The tongfengtai granulars and the diclofenac sodium capsule could significantly inhibit the uric acid sodium-induced rabbit synovial tissue IL-1β, IL-8, TNF-α, ICAM-1 expression, indicating that The tongfengtai granulars about the treatment of gout may be induced by inhibition of synovial tissue inflammatory cytokines IL-1β, IL-8, TNF-α, ICAM-1 release to achieve.Clinical researchDivides stochastically 108 examples of the acute uarthritis arthritis patient into three groups, the traditional Chinese medicine unioned western medicine groups (36 examples) gaved the tongfengtaigranular formulation and diclofenac sodium capsule, the traditional Chinese medicine groups (36 examples) gaved the tongfengtaigranular formulation, the western medicine groups (36 examples) gaved the diclofenac sodium capsule. Evaluated the treatment effects after five days, the traditional Chinese medicine unioned western medicine groups were convalesced 15 examples, the total effectiveness reaches 86.11%; The traditional Chinese medicine groups were convalesced 11 examples, the total effectiveness is 75%; The western medicine groups were convalesced 12 examples, total effectiveness 75%. traditional Chinese medicine united western medicine groups were higher than the total effective traditional Chinese medicine groups and western medicine groups. The overall effect of the clinical, especially in the preventing renal function, the clinical effects about traditional Chinese medicine combined Western medicine were significantly better than single-TCM or single-Western.SubtotaThe clinical research demonstrated that could significantly inhibit the uric acid sodium-induced rabbits'synovial tissue IL-1β, IL-8, TNF-α, ICAM-1 expression, indicated that the tongfengtai granulars about the treatment of gout may be induced by inhibition of synovial tissue inflammatory cytokines IL-1β, IL-8, TNF-α, ICAM-1 release to achieve. in the improving clinical symptoms, in the preventing early time kidney harm aspects, in especially, the curative effect that The traditional Chinese medicine combined Western medicine surpassesed just took the diclofenac sodium capsule. there were no statistical significance about clinical treatment effects from single-the tongfengtai granulars groups or single-the diclofenac sodium capsule groups. There were exactly clinical efficacy that the tongfengtai granulars in the treatment of acute gouty arthritis.