| Objective To investigate the effect of 48% chlorpyrifos on cholinesterase activity, the lipid peroxidation and Signal transduction pathway in rats. Methods Rats were randomly divided into low-dose (1/40LD50),middle-dose (1/20LD50), high-dose(1/10LD50) groups and Saline control group. The rats were treated by oral gavage for 28 days. The activity of whole blood cholinesterase were detected with ferric chloride colorimetric method, the contents of malondialdehyde (MDA) were detected with TBA in serum and brain tissue of the rats, the activity of superoxide dismutase (SOD) were detected with xanthine oxidase in serum and brain tissue of the rats, the activity of glutathione peroxidase (GSH.px) were detected with dithio-2 nitrobenzoic acid method in serum and brain tissue of the rats, the expression of Gq protein were detected with the Western blot in brain tissue, the expression of PLC were detected with the immunohistochemistry in brain tissue Results The activity of Whole blood cholinesterase degrades with increased exposure, the activity of Middle and high-dose group was statistically significant (P <0.01) compared with the control group,and was statistically significant (P<0.05) with the low dose group, The contents of MDA increases, was statistically significant (P<0.05) compared with the control group in the low dose group, and reduces in middle dose group, high-dose group.The activity of SOD (serum), GSH-px degrades with increased exposure, every dose group was statistically significant (P<0. 05)compared with the control group, every dose group was statistically significant (P <0.05)between the two groups; the activity of SOD in brain tissue degrades with increased exposure, every dose group was statistically significant (P<0.05)compared with the control group. The expression of Gq protein increases with increased exposure in brain tissue, every dose group was statistically significant (P<0.01) compared with the control group, every dose group was statistically significant (P<0. 01) between the two groups, The expression of PLC increases with increased exposure, every dose group was statistically significant (P<0.05) compared with the control group, every dose group was statistically significant (P<0.01) between the two groups, The results of PLC microscopic observation:every dose group have immunoreactive neurons, and the morphology and structure of the neurons is normal, cytoplasm is green, Nucleus is black vacuolar, the top dendritic of the neurons can be seen suddenly, Conclusions Chlorpyrifos injuries the body through lipid peroxidation at low doses probably, and by inhibiting the activity of cholinesterase,SOD,GSH-PX in the middle dose and high dose probably. the high expression of Gq, PLC may be involved nervous system pathological signal transduction in chlorpyrifos poisoning,,and this change may be the one of reasons of dysfunction of chlorpyrifos poisoning... |
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