The Relationship Between Nuclear Factor NF-κB Expression By Quartz Peroxidation And XuanWei Female Non-small Cell Lung Cancer

Objective: To study the relationship and biological significance among the depositions of crystalline silica in different lung tissues, nuclear factor NF-κB protein expression caused by oxidative damage and female non-small cell lung cancer (NSCLC) in Xuanwei.Method: PartⅠ: Transmission electron microscopy (TEM), selected area electron diffraction(SAED) and energy dispersive X-ray analysis (EDS) detect the occurrence of quartz in lung tissues of the following cases: experimental group , 18 cases of Xuanwei female lung cancer patients; control group A, 15 cases of Xuanwei male lung cancer patients; 16 cases of Non-xuanwei female lung cancer patients; control group B, 16 cases of Xuanwei male benign pulmonary diseases patients; 15 cases of Xuanwei female benign pulmonary diseases patients; 15 cases of Non-xuanwei female benign pulmonary diseases patients. Observing average particle size of quartz and local pathology in each group, the occurrence rates of quartz in different lung tissues as count data, using SPSS statistical software (R×Cχ2 test, if more groups would be a difference, then adjusting the test level compared to 2-sided) for statistical analysis.;.PartⅡ: Application of immunohistochemistry to detect the expression of NF-κB p65 protein in different lung tissues, and calculate the average optical density (OD), the results using SPSS statistical software (complete random design ANOVA -F test) for statistical analysis, with crystal size in the corresponding lung tissue for linear correlation analysis. Results: The test results of crystalline silica 1., the smallest particle size of quartz is 10.0nm×12.5nm, the maximum size of quartz is 1578.94nm×2500nm, average particle size is distributed around 200nm～450nm; quartz (<120nm) were often under the nuclear membrane or on the border of nuclear membrane and cytoplasmic in interstitial lung cells and alveolar typeⅡcells, with accumulation of sand-like polycrystalline compounds; The larger particles (>120nm) presented in alveolar, interstitial, or occasionally in the cytoplasm, there were a large number of collagen fibers, fibrosis in local occurrences. 2. For Xuanwei NSCLC groups, the occurrence rates of crystalline silica in cancer tissues, adjacent tissues and normal lung tissues showed no significant difference (P>0.05); 3. The lung tissues of Xuanwei female and male NSCLC were no difference in occurrence rate for crystalline silica; but both of them were above non-xuanwei female NSCLC lung tissures(P <0.01). 5. In benign lesions, there were the same overall distribution for the occurrence rates of crystalline silica, but they were significantly lower than Xuanwei female NSCLC (P<0.01).NF-kB protein expression level and the correlation analysis with crystalline silica: 1. In all NSCLC groups, the expression level of tumor tissue showed no significant difference (P> 0.05); In Xuanwei female NSCLC group, Xuanwei male NSCLC group, Non-xuanwei female NSCLC group, respectively the expression level of adjacent tissues and normal lung tissue decreased gradually (P <0.05). 2. Xuanwei female NSCLC tumor, normal lung tissue were significantly higher than those corresponding tissues in benign lesions group (P <0.02); in addition, Xuanwei female benign lesion group was the lowest (P <0.01). 3. In Xuanwei male and female NSCLC, the protein expression levels of most samples gradually were lower with the increase of quartz size(r = - 0.327, P = 0.028 <0.05; r = - 0.377, P = 0.005 <0.05), negative relationship); In control group B, the crystal size and NF-kB protein expression was not associated.Conclusion: 1. quartz has different degrees of deposition in the lung tissues of each group, the deposition in Xuanwei NSCLC was significantly higher than in patients of Non-xuanwei NSCLC and lung benign lesions, suggesting that quartz in Xuanwei lung cancer, especially Xuanwei female NSCLC plays an important role for the incidence of lung cancer. 2. The high expression of NF-kB protein in cancer tissues, the high expression of NF-kB protein in adjacent tissues and normal lung tissues in Xuanwei female NSCLC that was significantly higher than other group could be further studied as a diagnostic marker for lung cancer in Xuanwei and a possible target for treatment. 3. The smaller the crystal size was, the stronger the cytotoxicity of quartz was, leading to NF-kB protein highly expressed, which provides an important clue for further study of the pathogenesis of Xuanwei NSCLC.