| Objective To study the function of ras gene in the pathogenesis and development of human non-small cell lung cancer (NSCLC ) and compare the differences of ras gene alternations in NSCLC between Xuanwei and Kunming. Materials and Methods Samples of paraffin-embedded lung cancer tissues of 45 patients from Xuanwei and 45 patients from Kunming with NSCLC undergoing radical resection were analyzed for expression of ras protein and mutations of k-ras codon 12 by immunohistochemical staining, polymerase chain reaction-single strand conformation polymorphism and DNA direct sequencing. Genomic DNA was extracted from paraffin-embedded lung tissue by the methods of phenol-chloroform and DNA extraction and purification Kit. Results Among these 90 cases, 55 cases had ras protein expression and 28 cases had k-ras codon 12 mutations, there was no relevance between the expression of ras gene and the clinicopathological characteristics, and there was no relevance between the frequency of k-ras mutation and the clinicopathological characteristics either; Comparing the alternations of ras gene in two groups of NSCLC, the frequency of ras protein expression and the frequency of k-ras mutation in Xuanwei group were higher than that in Kunming group, but no statistically significant difference was found. Except that, therewere some statistically significant differences between two groups in terms of expression of ras protein in cases with stage I and cases with adenocarcinoma, but no statistically significant difference in k-ras 12 codon mutation was found between two groups. Conclusions These data suggest that the activation of ras gene is a commonly genetic event in the pathogenesis of NSCLC, the mutations of k-ras gene codon 12 are ubiquitous in two groups of NSCLC, there was statistically significant difference in ras protein expression in patients with stage I and in patients with adenocarcinoma between two groups.
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