The Expression And Significance Of ΔNp73 And P53 (Mutant Type) In Cutaneous Malignant Tumors Of Non-melanin

Objective To observe and contrast the expression of△N isoforms of p73(△Np73) and p53 (mutant type unless special explain)in cutaneous malignant tumors of non-melanin(squamous cell carcinoma, basal cell carcinoma and bowen disease), precancerous lesions(actinic keratosi), benign proliferating diseases(psoriasis,seborrheic keratosis) and nomal skin with immunohistochemistry. To discuss:the meaning of the expression of△Np73 in cutaneous malignant tumors of non-melanin; the actual value of△Np73 in malignant degree and prognosis of malignant tumors; The correlation of△Np73 and p53 in benign and malignant proliferating diseases.Methods The patients who diagnosed pathologically in dermatology department and pathology department accord with the diagnosis standard as well, moderately poorly differentiated squamous cell carcinoma(SCC) 60 cases, basal cell carcinoma(BCC) 20 cases, bowen disease(BD) 20 cases, actinic keratosi(AK) 20 cases, psoriasis (PS) 20 cases, seborrheic keratosis(SK) 20 cases were collected, meanwhile, we also collected nomal skin 10 cases from operating room of our department and dentofacial surgery. After all cases were stained with immunohistochemistry,we analyzed the differentia of positive cases in all kinds of diseases and the correlation of△Np73 and p53.Results All kinds of diseases were stained with p53. However,the positive proportion of p53 in cutaneous malignant tumors of non-melanin(SCC, BCC and BD) and precancerous lesions(AK) were significant higher than benign proliferating diseases(PS,SK) and nomal skin. The statistics difference was significant(P＜0.05). The difference between cutaneous malignant tumors of non-melanin(SCC, BCC and BD) and precancerous lesions(AK) was not significant(P＞0.05). All kinds of cutaneous malignant tumors of non-melanin(SCC, BCC and BD) and precancerous lesions(AK), some of benign proliferating diseases(PS) were stained with△Np73, others(SK,skin) were negative. the positive proportion of△Np73 in SCC and AK were significant higher than other diseases. The statistics difference was significant(P＜0.05). The difference between SCC and AK was not significant(P＞0.05). Both the statistics difference between the positive proportion of△Np73 in all kinds of diseases and anatomic sites (Exposure site and Non-exposure site), gender, age were not significant(P＞0.05).The positive correlation of△Np73 and p53 in SCC was observed(rs=0.348, P=0.007). There were no correlation of△Np73 and p53 in other diseases(P＞0.05).Conclusion The expression of p53 is helpful to diagnosis and discrimination in benign and malignant proliferating diseases, but the specificity of diagnosis and discrimination in cutaneous malignant tumors of non-melanin and precancerous lesions is not good; The expression of△Np73 is specific in diagnosis and discrimination to other diseases of SCC and AK, but is not helpful to pathology classification of SCC; The correlation of△Np73 in all kinds of diseases and age, gender,anatomic sites were not observed; The relationship of△Np73 and p53 in SCC maybe tightness because of the correlation of expression of△Np73 and p53 is obviously; Combination of△Np73 and p53 is more useful than separateness in diagnosis and discrimination of benign and malignant proliferating diseases; The complicated relationship of△Np73 and cutaneous malignant tumors of non-melanin is not clear still, therefore, we need more cases research with large samples and detailed clinical data to explain it.