| Objective:Observing the effects of recuperating-heart granule which can tonify Qi,nourish blood,stabilize the heart and tranquilize the mind on the expression of Matrix metalloproteinase-9 in the arteria carotis communis atheroma sclerotic plaque of rabbit model of similar unstable angina to investigate its mechanism of action and to provide a reliable theoretic basis for this therapy.Methods:Aortic wall injuries were introduced using an intravascular balloon in rabbits before the 28 rabbits were fed on a high fat diet for 8 weeks. To confirm forming atherosclerosis plaque by color ultrasonography,then they are randomly divided into model control group,recuperating-heart granule group, Cedocard group and atorvastation group.There are 7 rabbits in every group. Check another seven as a blank group.Given a two-week drug treatment.At the end of week 10,to copy the model of similar unstable angina pectoris by using the method of pharmacological triggering.To observe the thickness and blood flow rate of arteria carotis communis by body surface chromatic color ultrasonography and to observe pathological changes of blood vessel by HE dyeing;To detecte the expression of Matrix metalloproteinase-9 by immunohistochemistry.Results:1.According to the results of body surface ultrasonography,at the end of 8 weeks,compare with blank group,every group emerge that arteria carotis communis IMT obviously thicken,PI and RI obviously addition(P＜0.01 or P＜0.05),but Vs of every group accelerate nubilously.At the end of 10 weeks, every therapeutic group IMT,PI,RI decreased,compared with model control group,they are considered statistically significant(P＜0.01 or P＜0.05),but in Cedocard group IMT descent obsoletly(P＞0.05);difference in recuperating-heart granule group and atorvastation group is nonsense(P＞0.05).2.Pathology resules indicated,besides black control group,every group emerge that vascular endothelial cell damaged,endangium thicken,fatty deposition obviously,fatty substance plaque formed.Each treatment group's arteria carotis communis endothelial cell decrease lightly,endomembrane thicken lightly,fatty deposition decrease under endomembrane,arterial media vascular smooth muscle cells proliferation,adventitia fibrosis and chronic inflammation cell infiltrated.3.According to the results of MMP-9,at the end of 10 weeks,The expression of MMP-9 at the model control group increases obviously, c0mpared with the blank group,it was considered statistically significant (P＜0.01).The expression of MMP-9 at recuperating-heart granule group and Atorvastation group decrease obviously,compared with the model control group,it was considered statistically significant(P＜0.01),and they have the similar effects;Cedocard group doesn't decrease MMP-9Conclusion:Recuperating-heart granule which on the theory of tonify Qi,nourish blood,stabilize the heart and tranquilize the mind has the definite preventive and therapeutic effect to rabbit model of similar unstable angina,it can decrease the expression of MMP-9,reduce thickening extent and improve pathomorphism of the blood vessel,thus it can restrain endangium hyperplasia,hamper smooth muscle cell migration and plaque disruption.So it can prevent and cure unstable angina,...
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