Effect Of Recuperating-Heart Granule On Serum IL-6 Of The Rabbit Model Of Similar Unstable Angina

Objective:To observer the effects of recuperating-heart granule that it can to tonify Qi and nourish blood,stabilize the heart and calm the mind on rabbit model of similar unstable angina that the expression of serum Interleukin-6(IL-6),to investigate its mechanism of action at level of molecular biology,to provide a reliable theoretic basis for this therapy.Methods:Aortic wall injuries were introduced using an intravascular balloon in rabbits before the 28 rabbits were fed on a high fat diet for 8 weeks.To confirm forming atherosclerosis plaque by color ultrasonography,then they were randomly divided into model control group,recuperating-heart granule group,Cedocard group and atorvastation group.There were 7 rabbits in every group.Check another 7 rabbits as a blank group.Given a two-week drug treatment.At the end of week 10,to copy the model of similar unstable angina pectoris by using the method of Pharmacological triggering.To determine content change of blood serum IL-6 by ELISA measured;to investigate the thickness and blood flow rate of arteria carotis communis by body surface chromatic color ultrasonography and to observe pathomorphism change of arteria carotis communis by HE dyeing.Results:1.According to the result of IL-6:At the end of 8 weeks,compare with blank group,IL-6 obviously increase in each group,it was considered statistically significant(P<0.01).At the end of 10 weeks,compare with control model group,IL-6 obviously decline in therapeatic group,it was considered statistically significant(P<0.01);compare with other therapeatic group,IL-6 obviously decline in recuperating-heart granule group,it was considered statistically significant(P<0.05),But difference in Cedocard group and atorvastation group is nonsense(P>0.05).2.According to the results of body surface ultrasonography:At the end of 8 weeks,compare with blank group,every group emerge that arteria carotis communis IMT obviously thicken,PI and RI obviously addition,it was considered statistically significant(P<0.01,P<0.05),but Vs of every group accelerate nubilously,it was considered no statistically significant (P>0.05).At the end of 10 weeks,compare with model control group,every therapeatic group IMT PI and RI obviously decline,it was considered statistically significant(P<0.05),IMT of recuperating-heart granule group and atorvastation group obviously decline,it was considered statistically significant(P<0.05),Cedocard group IMT descent obsoletly,it was considered no statistically significant(P>0.05).3.Pathology research indicated:Besides black group,every group emerge that vascular endothelial cell damaged,endangium thicken,fatty deposition obviously,fatty substance plaque formed.Compared with model control group,each treatment group's arteria carotis communis endothelial cell decrease lightly,endomembrane thicken lightly,fatty deposition decrease under endomembrane,arterial media vascular smooth muscle cells proliferation,adventitia fibrosis and chronic inflammation cell infiltrated lessen.Conclusion:Recuperating-heart granule which can tonify Qi and nourish blood,stabilize the heart and calm the mind on the model of similar unstable angina can highly lower the level of serum IL-6 of rabbit model unstable angina pectoris,Recuperating-heart granule can be significantly improved abnormal atherosclerosis pathomorphology of the rabbit model,can restrain endangium hyperplasia.Maybe this is the one of mechanism of action to prevention and cure unstable angina.